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Electrophoresis, in DNA sequencing

Features. A series of boxed and highlighted Features are found throughout the text. These essays contain interesting applications of analytical chemistry to the modern world, derivation of equations, explanations of more difficult theoretical points, or historical notes. Examples include Breath Alcohol Analyzers (Chapter 7), Antioxidants (Chapter 20), Fourier Transform Spectroscopy (Chapter 25), LC/MS and LC/MS/MS (Chapter 32), and Capillary Electrophoresis in DNA Sequencing (Chapter 33). [Pg.1173]

Resolution in capillary gel electrophoresis of DNA sequencing was shown to be directly proportional to the product of the number of bases and the relative peak distance, i.e., to the mean separation of peaks.43 Reformulation of the treatment of the capacity factor has been used to simplify and clarify the interpretation of the separation factor in electrophoresis.44 Peak... [Pg.430]

Mihindukulasuriya SH, Morcone TK, McGown LB (2003) Characterization of acridone dyes for use in four-decay detection in DNA sequencing. Electrophoresis 24 20-25... [Pg.36]

PAG electrophoresis is an important technique in many areas. In DNA sequencing it provides a fundamental method of separation, and in protein studies it is important both in the form described here and also in conjunction with the detergent, sodium dodecyl sulphate (SDS), giving a valuable method for assessing the relative molecular mass of a protein. [Pg.138]

Since protocols for electrophoresis used in DNA sequence analysis are given, for example, by Sambrock et al., these specialized methods are not outlined in this chapter. [Pg.45]

Once the sample preparation is complete, the analysis is carried out by an instrument of choice. A variety of instruments are used for different types of analysis, depending on the information to be acquired for example, chromatography for organic analysis, atomic spectroscopy for metal analysis, capillary electrophoresis for DNA sequencing, and electron microscopy for small structures. Common analytical instrumentation and the sample preparation associated with them are listed in Table 1.1. The sample preparation depends on the analytical techniques to be employed and their capabilities. For instance, only a few microliters can be injected into a gas chromatograph. So in the example of the analysis of pesticides in fish liver, the ultimate product is a solution of a few microliters that can be injected into a gas chromatograph. Sampling, sample preservation, and sample preparation are... [Pg.2]

Polyethylene oxide (PEO) and polyvinylpyrrolidone (PVP) solutions both proved to be good separation matrices in capillary electrophoresis-based DNA sequenc-... [Pg.83]

Mural R. J., Mann R. C., Uberbacher E. C. (1990) Pattern recognition in DNA sequences The intron-exon junction problem. In The first International Conference on Electrophoresis, Supercomputing and the Human Genome. (Cantor C. R., Lim... [Pg.126]

D Schmalzing, L Koutny, O Salas-Solano, A Adourian, P Matsudaria, D Ehrlich. Recent developments in DNA sequencing by capillary and microdevice electrophoresis. Electrophoresis 20 3066-3077, 1999. [Pg.379]

The above described PCR can only amplify the number of copies of a DNA molecules in the sample. In order to know what DNA or specific DNA sequence it is, however, one has to perform additional analyses, such as using gel electrophoresis or DNA sequencing method to find the answer. A direct DNA identification method, called the real-time PCR, combining both the PCR with fluorescent detection, is most useful in this regard. [Pg.379]

The same reaction is done with each of the dideoxynucleotides. The DNA fragments are then separated by gel electrophoresis on a DNA sequencing gel. The four reactions are placed in four wells, side by side, on the gel. Following electrophoresis, the DNA sequence can be read directly from the gel, as shown in Figure 24.26. [Pg.748]

In Chapter 25, we describe the use of gel electrophoresis for separation of nucleic acids in DNA sequencing. [Pg.631]

Proteins, amino acids, and carbohydrates have all been separated in minimum times by CZE. In the case of neutral carbohydrates, the separations arc preceded by formation of negatively charged borate complexes. Protein mixtures can be separated, as illustrated in Figure. 30-13, Capillary gel electrophoresis is widely used in DNA sequencing as discussed in the next section,... [Pg.877]

Quesada, M.A., Replaceable polymers in DNA sequencing by capillary electrophoresis. Curt Opin. BiotechnoL, 8, 82, 1997. [Pg.506]

Wei, W. and Yeung, E.S., Improvements in DNA sequencing by capillary electrophoresis at elevated temperature using poly(ethylene oxide) as a sieving matrix, J. Chromatogr. B, 745, 221, 2000. [Pg.509]

The field of chemical cytometry is in its infancy, and it is clear that there is much work to be done before the technology is widely used. First, instrumentation throughput must be increased. Flow cytometry today can process a 100,000 cells/s. While chemical cytometry will not produce similar throughput, it is reasonable to expect the technology to process 10,000 cells/day in onedimensional electrophoresis and perhaps 1000 cells/day in two-dimensional electrophoresis. Such instrumentation will likely resemble the multiple-CE systems that have become ubiquitous in DNA sequencing. - ... [Pg.627]


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See also in sourсe #XX -- [ Pg.1176 ]




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