Agarose gel electrophoresis, of DNA

Eor example, the technique of Southern blotting was developed (68) for use with agarose gel electrophoresis of DNA fragments. Southern blots are designed to detect specific sequences of DNA. After electrophoresis is complete, the DNA is denatured and the single stranded DNA transferred to the specially prepared nitrocellulose paper. The nitrocellulose is then incubated with radioactive RNA or DNA complementary to those DNA sequences of interest. After the nitrocellulose has been sufftciendy incubated with the radioactive complementary DNA, autoradiography is used to identify the fragments of interest. 

Flg.(6). Morphological appearance of PC-12 cells treated for 24 h with vehicle alone (panel A), TNF-a (500 units/ml) alone (panel B), or TNF-a (500 units/ml) plus 10 pM crocin (panel C). In panel D, intemucleosomal DNA fragmentation was detected by agarose gel electrophoresis of DNA extracted from cells treated for 24 h. 

Figure 2. Agarose gel electrophoresis of DNA Lane /, none lanel, H2O2 + Fe " lane 3, H2O2 + Fe + (-)-epigallocatechin 3-O-gallate (2.5 pg/ml) lane 4, H2O2 + Fe + (-J-epigallocatechin 3-O-gallate (5 pM4, marker 4 M6, marker 6. (Copyright 1998 American Chemical Society.)...

Fig. 4. Agarose gel electrophoresis of transcribed mRNA in vitro. Left lane DNA marker III digest. Middle and right lanes mRNA of yeast ubiquitin mRNA at...

Figure 7.12 Agarose gel electrophoresis of the PCR product from the recombinant DNA. Lane 1, PCR product from the recombinant DNA lane 2, PCR product from the mixture without the enzymatic ligation M, 200 base pair ladder.

Air-dry the DNA and resuspend in sterile TE. Adjust vol to 100 ng/mL. (DNA will be diluted from this stock to the appropriate concentration on the day of injection.) Confirm the identity, purity, and concentration of the DNA fragment by agarose gel electrophoresis of 500 ng with standards of known concentration. 

Day 4 Restriction Endonuclease Digestion and Agarose-Gel Electrophoresis of Plasmid DNA... 

Fig. 2 Effect of 4-1rifluoromeIhylimidazoles on DNA fragmentation for A 6h and B 24h (assayed by agarose gel electrophoresis). M DNA size marker...

Fig. 8.1. (a) DNA forms form I, supercoiled form II, relaxed form III, linear, (b) Schematic representation of agarose gel electrophoresis of forms I, II and III. 

Fig. 9. Positive supercoiling of SSVl DNA. Panels A and B show two-dimensional agarose gel electrophoresis of SSVl DNA isolated from cells of Sulfolobus shibatae, (A) before and (B) after UV induction (for methods see refs. [39,98]). The left-hand branch of the arch visible in A corresponds to negatively supercoiled DNA, the top of the arch corresponds to relaxed DNA and the right-hand branch corresponds to positively supercoiled DNA. The upper bands in A and B correspond to form II (open circular) and the middle band in B corresponds to form III (linear SSVl) (pictures courtesy of G. Mirambeau). Panel C shows a one-dimensional agarose gel electrophoresis of SSVl DNA isolated...

Fig. 2. Agarose gel electrophoresis of apoptotic DNA ladder formed by the incubation of cells with ceramide STPP liposomes. The cells were incubated with the various liposomal preparations for 18 h at a ceramide concentration equivalent to 25 pM, followed by DNA extraction and gel electrophoresis. Lane a ceramide liposomes lane b ceramide STPP liposomes lanec untreated...

FIGURE 2.4 Agarose gel electrophoresis of PCR products. Along with the PCR products, reference DNA fragments are separated to estimate the size of fragments amplified by PCR. 

Uses Buffer for gel electrophoresis prep, of agarose gels separation of DNA in agarose and polyaacrylamide gels... 

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