DNA cloning experiments

Rat genome, 41 "Rational polytherapy," 233 Rawls, John, 80 Recombinant DNA cloning experiments with, 30 Cohen-Boyer patent on, 73 Recombination events, 57-58 Reeder, C. E., 229... 

Cohen, Chang, Boyer, and Helling reported the first DNA cloning experiments. 

DNA cloning experiments combine these technologies with a few additional tricks to isolate single copies of a gene and then produce billions of copies. To produce... 

Contig A group of pieces of DNA, derived from a cloning experiment (often a series ( ESTs, see below), that represent overlapping regions of a chromosome... 

Figure 2.3 Metagenomic cloning experiments. Isolation of genomic DNA directly from environments (soil, plants, mixed environments or thermal-vent worms are the examples Illustrated here) can recover DNA fragments which could encode for enzymes. The DNA fragments can be ligated to plasmids or DNA linkers, and then subjected to functional screening (expression cloning) and/or sequence analysis. Amplification by PCR can sometimes be used to yield libraries enriched with clones containing selected sequence motifs relating to families of enzymes...

Identification of the Desired Passenger Sequence in Plasmid Cloning Experiments. The objective of recombinant DNA... 

L. Perez-Pons and E. Querol, Biochem. Educ. 24, 54-56 (1996). A laboratory experiment illustrating basic principles of DNA cloning and molecular biology techniques. J. Sambrook, E. Fritsch, and T. Maniatis, Molecular Cloning, A Laboratory Manual, 2nd ed. (1989), Cold Spring Harbor Laboratory (Cold Spring Harbor, NY), Vols. I, II, and III, pp. 1.21-1.52. Techniques in recombinant DNA. 

Experiment 14 introduced students to some principles and techniques involved in recombinant DNA research. Specifically, the experiment outlined the replication, isolation, and analysis of bacterial plasmid vehicles for molecular cloning experiments. This experiment describes another tool that is essential in hybrid plasmid construction and analysis-restriction enzyme action. The procedures introduced here have also found widespread use in the analysis and characterization of all DNA molecules. 

DNA serves as a blueprint for the individual, controlling, among other aspects, the production of highly specific proteins. In sexual reproduction, the DNA of the offspring is derived from the DNA of both parents, and so serves as the material basis of inheritance and of evolution. Cloning experiments, from Dolly the sheep onward genetically modified crops the possible treatment of inherited diseases—in these and other ways, an understanding of the chemical basis of reproduction and in-... 

It is advisable to have three transformation tubes for every cloning experiment. One is test sample L tube, second is positive control C tube, and third is the no DNA control. Control C tube will tell the researcher about the transformation efficiency of the competent cells. The no DNA control should not have any colonies on the LB-ampicillin plate. It indicates that there was no contamination of the host E. coli cells and the ampicillin drug is acting as expected. The degradation of ampicillin in the plates is indicated if the no DNA control plate shows a lot of colonies. 

---