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Clones. DNA

Grunstein, M., and Hogness, D. S., 1975. Colony hybridization A specific method for the isolation of cloned DNAs tliat contain a specific gene. Proceedings of the National Academy of Sciences U.S.A. 72 3961—3965. Article describing die colony hybridization technique for specific gene isolation. [Pg.423]

Gene therapy uses cloned DNA in adeno or retroviral vectors to transduce host cells for longterm therapy of certain genetic disorders with defined... [Pg.265]

The cloned DNA can be used as a probe in one of several types of hybridization reactions to detect other related or adjacent pieces of DNA, or it can be used to quantitate gene products such as mRNA. [Pg.412]

One usually tries to adjust experimental conditions so that only one plasmid or phage is introduced into a single cell. When that cell replicates on a plate of nutrient agar, it will produce millions of cells until a colony becomes visible to the naked eye. If cells are sufficiently diluted before they are spread onto the plate, each colony will consist of a clone derived from a single ancestral cell. If each cell originally contained only one copy of the phage or plasmid vector, each colony will contain recombinant DNA that is homogeneous. That is, each colony will contain cloned DNA. [Pg.250]

PHA depolymerases, 20 253, 256 pH adjusting agents, 12 61-62 Phage cloning, DNA isolation for,... [Pg.690]

Replicate. 1 Recover cloned DNA in replicated plasmid by using RE. Recover replicated DNA lysing E. coli. Clone in plasmid. 4 Recover. 4 Package into phage heads. 4 Clone in E. coli. [Pg.8]

Simpson RT, Thoma F, Brubaker JM (1985) Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones a model system for study of higher order structure. Cell 42 799-808 Sugiyama S, Yoshino T, Kanahara H, Kobori T, Ohtani T (2003) Atomic force microscopic imaging of 30 nm chromatin fiber from partially relaxed plant chromosomes. Scanning 25 132-136 Sugiyama S, Yoshino T, Kanahara H, Shichiri M, Fukushi D, Ohtani T (2004) Effects of acetic acid treatment on plant chromosome structures analyzed by atomic force microscopy. Anal Biochem 324 39 4... [Pg.28]

Rousset M, Casalot L, Rapp-Giles BJ, et al. 1998. New shuttle vectors for the introduction of cloned DNA in Desulfovibrio. Plasmid 39 114-22. [Pg.97]

Cloning DNA restriction fragments is usefijl in the following applications ... [Pg.81]

Once the corresponding colony has been identified, a sample can be used to inoculate a large broth culture from which one can isolate the cloned DNA or the recombinant protein. [Pg.86]


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See also in sourсe #XX -- [ Pg.152 , Pg.153 ]




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A method for sequencing single stranded cloned DNA in both directions by the dideoxynucleotide-chain termination procedure

Cloning and identification of genes in DNA

Cloning of DNA

Cloning of DNA fragments

Cloning of Recombinant DNA

Complementary DNA cloning

DNA cloning

DNA cloning

DNA cloning experiments

DNA into a cloning vehicle

Expression of Cloned DNA

Finding an Individual Clone in a DNA Library

Plasmids Are Used to Clone Small Pieces of DNA

Recombinant DNA cloning

Sources of DNA for Cloning

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