DNA-carbohydrate conjugates

Investigations on the interactions of glycosylated nucleotide sequences with proteins and the effective cell-specific delivery of DNA-carbohydrate conjugates... 

The second main area of application results from the high selectivity of DNA-carbohydrate conjugates for cell recognition and increased specific cellular uptake due to stability against nucleases. 5-Neoglycoconjugates have revealed excellent cell-type specificity and cellular uptake in vitro and in vivo [41 5]. For instance, Hangeland et al. [41] demonstrated that the neoglycoconjugate... 

This report was the first of its type to show the ability of such a large covalently linked carbohydrate (neomycin) to aid in RNA-DNA hybrid complex-ation. N-Neo-conjugate and a 7-base-long RNA sequence present in a-sarcin loop can form a stable hybrid duplex. The stability of a hybrid duplex with addition of one neomycin equivalent is comparable to the stability of a hybrid duplex where the DNA has a covalent bond to neomycin. The presence of a single mismatch decreases the melting temperature of both neomycin-conjugated... 

Fig. 7.21. Enzyme coupling via p-benzoquinone (pBQ) activation (pBQ also reacts with carbohydrates) and coupling to a basic polymer (I). At pH 6, only one of two potential sites of pBQ reacts, whereas at pH 8 the second site is activated. The complex is conjugated to denatured DNA using glutaraldehyde. Alternatively, haptenated basic polymers can be linked by 1,2,7,8-diepoxyoctane (carcinogenic ) to DNA (II).

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