DNA conjugates

Rogers F.A., Vasquez K.M., Egholm M., Glazer P.M. Site-directed recombination via bifiinctional PNA-DNA conjugates. Proc. Nad. Acad. Sci. USA 2002 99 16695-16700... 

Yokoyama K, Taira S (2005) Self-Assembly DNA-Conjugated Polymer for DNA Immobilization on Chip. 262 91-112 Yoshikawa I, see Araki K (2005) 256 133-165... 

Bentolila LA, Weiss S (2006) Single-step multicolor fluorescence in situ hybridization using semiconductor quantum dot-DNA conjugates. Cell Biochem Biophys 45 59-70... 

Another coupling method, i.e. cross-linking or entrapment in polymeric films, which has been used to create a more permanent nucleic acid surface, is described in some chapters (e.g. conductive electroactive polymers for DNA immobilization and self-assembly DNA-conjugated polymers). One chapter reviews the basic characteristics of the biotin-(strept)avidin system laying the emphasis on nucleic acids apphcations. The biotin-(strept)avidin system can be also used for rapid prototyping to test a large number of protocols and... 

Self-Assembly DNA-Conjugated Polymer for DNA Immobilization on Chip... 

Ho HA, Bera-Aberem M, Leclerc M. Optical sensors based on hybrid DNA/conjugated polymer complexes. Chemistry 2005 11 1718-1724. 

Cai et al. [37] SW CNT-1-plasmid DNA, with nickel under the influence of a magnetic field High efficiency of transduction of SW CNT-DNA conjugates in lymphoma cells (Ball 7 B-lymphoma)... 

Stachel, S.E. Zambryski, P.C. (1986). Agrobacterium tumefaciens and the susceptible plant cell a novel adaptation of extracellular recognition and DNA conjugation. Cell 47, 155-7. 

Fig. 1. Comparison of enzyme-linked immuno sorbent assay (ELISA, left) and immuno-polymerase chain reaction (IPCR, right). During ELISA, an antibody-enzyme conjugate is bound to the target antigen. The enzyme converts a substrate in solution to a detectable product. In IPCR, the antibody-enzyme conjugate is replaced by an antibody-DNA conjugate. The subsequent addition of a DNA polymerase enzyme (e.g., Taq), nucleotides and a specific primer pair uses the antibody-linked DNA marker sequence as a template for amplification of the DNA. The PCR product is finally detected as an indicator of the initial amount of antigen.

IgG from several species (mouse, rabbit, goat, and human), Aviscumin, research antibody in cell culture media 2003 A, B, C Factor 100-1000 (IPCR 0.1-0.01 amol [50-500 fg/mL] IgG, 40 pg/ mL Aviscumin in serum, 100 pg/ mL research antibody) Adler et al. [62], real-time IPCR (Section 2.2.3), internal competitor (Section 2.3.6), commercial antibody-DNA conjugates, AbiPrism 7000 sequence detector (Applied Biosystems)... 

The development of easy accessible complete antibody DNA conjugates for single-step incubation is nevertheless the most promising approach for adapting IPCR to the needs of robust and fast clinical routine application. 

For demonstrating the properties of the multiplex IPCR and covalent antibody-DNA conjugates (see Section 2.1.3), a number of hormones such as human thyroid stimulating hormone or chorionic gonadotropin were simultaneously detected by the workgroup of Ebersole [38, 41]. In the... 

Fig. 10. Different techniques for competitive immuno-polymerase chain reaction of small-molecule compounds. (A) Using biotinylated haptens and a DNA-streptavidin nanocircle conjugate [105], hapten-DNA conjugates were synthesized and used for a competitive assay in a sample containing free hapten and capture antibody-coated surfaces [92]. (B) Hapten-coated microplates were simultaneously incubated with a sample containing free hapten and a hapten-specific antibody. Following competitive coupling, the immobilized antibody was subsequently detected by a species-specific antibody-DNA conjugate [93, 94].

---