Conjugated DNA

J. H., Hoogerbrugge, J. W., Vreeberg, M. T. M., Baarends, W. M., Bootsma, D., Grootegoed, j. A., and Hoeijmakers, j. H. j. Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification. Cdl 1996, 86, 799-810. 

Ulp2 S. cerevisiae increased SUMO conjugates, DNA repair defective desumoylating enzyme [123]... 

Artificial peptides, such as GALA and JTS-1, and the sequence of the /V-terminal segment of VP1 of HRV2 rhinovirus, have also been found to enhance the transfection efficiency of polylysine conjugates/ DNA complexes (Table 16.1). These peptides, which contain acid residues (mainly glutamate) were shown to induce membrane fusion and permeabilization at pH lower than 6. Permeabilization and fusion activities... 

The direct detection of DNA via conformational changes of ferrocene-conjugated DNA stem-loop (or hair-pin) structure onto electrode surface induced by the hybridization was also proposed (Fig. 11.1c) [4, 20]. The conformational change induces the displacement of ferrocene molecules from the electrode surface, resulting in a drop in peak redox current measured by cyclic voltametry. The detection limits were 115 fM for 24-base oligonucleotide [20] and 10 pM for 17-base oligonucleotide [4], respectively. Furthermore, the difference between... 

Fig. 3 Distribution of injected DNA vaccines. A rhodamine-conjugated DNA vaccine was injected into a tibialis anterior muscle of a mouse shown by light (panel A) and fluorescence (panel C) microscopy ( 5x magnification). A longitudinal section of the muscle is shown in panel B ( 250x magnification), demonstrating the presence of DNA in cells between the muscle fibers. Panel C shows the phagosomal location of the plasmid DNA (in red) within the cells isolated from the injected tissues ( 2500x magnification). (See Color Plate p. xxii).

Bailly, V., Lauder, S., Prakash, S., and Prakash, L. (1997b). Yeast DNA repair proteins Rad6 and Radl8 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activities. J. Biol Chem. 272, 23360-23365. 

Fluorescent NeutraLite avidin (various fluorescent conjugates) DNA counter-staining dyes (DAPl, Sytox, OliGreen, Propidium iodide, etc.) catalog numbers available through searchable web site international distributors on web site]... 

Chitosan-Conjugated DNA Nanoparticle Delivery Systems for Gene Therapy... 

In this review, we evaluated the preparation and application of chitosan-conjugated DNA and siRNA nanoparticles for gene delivery applications in detail. 

FIGURE 9.17 Selective release by laser irradiation of DNA conjugated to nanocapsules (ovals) and nanobones (bones). Exposure to X = 800 run irradiation (left) melts the nanocapsules only and selectively releases the conjugated DNA (dye-labeled, triangles). Exposure to X = 1100 nm irradiation (right) melts the nanobones only and selectively releases the conjugated DNA (dye-labeled, stars). Reproduced with permission from [135]. Copyright 2009 American Chemical Society. 

Jayakumar R, Chennazhi KP, Muzzarelli RAA, Tamura H, Ntiir SV, Selveunurugan N. Chitosan conjugated DNA nanoparticles in gene therapy review ruticle. Carbohydr Polym. 

Keywords Conjugates DNA Micelles Oligonucleotide Self-assembly ... 

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