Digestion methods microwave

Compared to enzyme digestion methods, microwave heating demonstrated more intense immunoreactivity of estrogen and progesterone in breast cancer tissues fixed with methacam (60% methanol, 30% chloroform, and 10% acetic acid) (Oyaizu et al., 1996). 

Evaluate whether the microwave digestion method yields acceptable results in comparison with the standard digestion method. 

Comparative studies were performed to evaluate microwave digestion with conventional sample destmction procedures. These included the analysis of shellfish, meats, rocks, and sods. Generally, comparable accuracy at much shorter digestion time was found for the MAE vs the classical digestion method (39). 

COMBINED ENZYME DIGESTION AND MICROWAVE HEATING ANTIGEN RETRIEVAL METHOD... 

Parr, J. F., Dolic, V., Lancaster, G, and Boyd, W. E. (2001). A microwave digestion method for the extraction of phytoliths from herbarium specimens. Rev. Palaeobot. Palynol. 116, 203-212. 

Adequate digestion methods are important in the determination of all metals, including aluminum. Que Hee and Boyle (1988) showed that Parr bomb digestions were always superior to hot plate digestions for many elements, including aluminum, in feces, liver, and testes. Microwaving in closed vessels produced lower aluminum recoveries in liver than Parr bomb digestions. The Parr bomb values for citrus leaves were within 5% of the NBS certified values. 

Niemela, M., H. Kola, P. Peramaki, J. Piispanen, and J. Poikolainen. 2005. Comparison of microwave-assisted digestion methods and selection of internal standards for the determination of Rh, Pd and Pt in dust samples by ICP-MS. Microchim. Acta 150 211-217. 

Eilola, K. and P. Peramaki. 2003. Development of a modified medium pressure microwave vapor-phase digestion method for difficult to digest organic samples. Analyst 128 194—197. 

M. D. Mingorance, M. L. Perez-Vazquez, M. Lachica, Microwave digestion methods for the atomic spectrometric determination of some elements in biological samples, J. Anal. Atom. Spectrom., 8 (1993), 853-858. 

Takenaka, M., Kozuka, S., Hayashi, M., Endo, H. Determination of ultratrace amounts of metallic and chloride ion impurities in organic materials for microelectronics devices after a microwave digestion method. Analyst 122, 129-132 (1997)... 

Xu, L.Q., Shen, W.X. Study on the PTFE closed-vessel microwave digestion method in food elemental analysis. Fresenius Z. Anal. Chem. 332, 45 7 (1988)... 

Eilola, K., Peramaki, P. Microwave heated vapor-phase digestion method for biological sample materials. Fresenius J. Anal. Chem. 369, 107-112 (2001)... 

Another application area in microwave technology is the use of microwave irradiation for the enzymatic digestion of proteins [106]. As discussed in the early part of this chapter, enzymatic cleavage to produce smaller peptide fragments of protein samples is an important step in structural characterization of proteins [24]. Traditional enzymatic digestion method usually takes several hours, whereas microwave-assisted digestion occurs in minutes. The initial... 

Sample Preparation Using Microwave Acid Digestion Method... 

Sample preparation using the bombing combustion method has an advantage over the microwave digestion method in that it does not require very strong multi-acidic solutions... 

To monitor these concentrations of stabilisers for precise, accurate measurements a method must be available that must detect levels as low as 600 ngg-1 and as high as 5000 ngg-1. The allowed tolerance can vary between 20% for low levels to 10% for higher levels without affecting the performance of the product. The lower concentration would be expected to cure faster than those containing higher concentrations of stabiliser. The different level of stabiliser is dictated by the application of these adhesives and must be formulated under very controlled conditions. ICP-OES is an ideal tool to monitor the metal type stabilisers using sample dilution, bomb combustion or microwave acid digestion methods. 

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