Diethylaminoethyl-dextran

Newer 2-diethylaminoethyl ethers, such as 0-( 2-diethylaminoethyl )-agarose gels," are useful as media for electrophoresis and immunoelec-trophoresis, and the 2-diethylaminoethyl derivative of O-(2-hydroxy-propyl ) cross-linked dextran (Sephadex LH20) is an effective, lipophilic anion-exchanger. The relative stiffness of the molecular chain of 0-(2-diethylaminoethyl) dextran has been estimated from measurements of viscosity at various ionic strengths, and compared with that of other polysaccharide polycations and polyanions. 0-(2-Diethylaminoethyl) dextran itself is claimed to be toxic." ... 

McCutchan, J. H. and Pagano, J. S. (1968). Enchancement of the infectivity of simian virus 40 deoxyribonucleic add with diethylaminoethyl-dextran. J. Natl. Cancer Inst. 41,351-357. 

Cre CV1 DEAE-dextran DHFR DMSO DNA DOPE DOSPA recombinase (cyclization recombination) African green monkey kidney cells diethylaminoethyl-dextran dihydrofolate reductase dimethylsulf oxide deoxyribonucleic acid dioleoylphosphatidyl ethanolamine 2,3-dioleoyloxy-/V-[2(sperm inecarbox-amido)ethyl]-/V,TV-dimethyl-1-... 

Similar delivery systems have been designed using other cationic polymers, including lipopolyamines (161), amphipathic peptides (162), polylysine lipids (163), cholesterol (164), quaternary ammonium detergents (165), polyamidoamines called dendrimers, polyethylenimines or chitosan, diethylaminoethyl dextran (DEAE dextran), and polybrene. 

Other chemical inducers, such as pyran copolymers, tilorone, diethylaminoethyl dextran, and heparin, also have been used. Tilorone is an effective inducer of interferon in mice, but it is relatively ineffective in humans. Initial use of interferon and its inducers instilled intranasally after rhinovirus exposure was successful in the prevention of respiratory diseases. The clinical success of interferon and its inducers has not yet been established, although they may play a significant role in cell-mediated immunity to viral infections and cancer. Disadvantages of interferon use include unacceptable side effects, such as fever, headache, myalgias, leukopenia, nausea, vomiting, diarrhea, hypotension, alopecia, anorexia, and weight loss. 

Some single-strand RNAs, for example poly I, poly C, yeast RNA when in the presence of relatively large amounts of polybasic substances such as diethylaminoethyl dextran, poly-L-lysine, protamine, histone or methylated albumin, could induce interferon production in cells, but much higher concentrations of RNA were required than of the double-stranded variety [121, 122]. There is some recent evidence that a single-stranded virus RNA will induce interferon even without potentiation [123]. 

In a variation of the above process, a water soluble monomer A is adsorbed on water-immiscible solvent droplets dispersed in the aqueous solution of A and then polymerized to form closed shell structure. For example, cross-linked polysaccharide capsules with diameters ranging from 200 nm to several microns and wall thicknesses of several tens of nanometers have been fabricated by interfacial polymerization of methacrylated N,N-diethylaminoethyl dextran (DdexMA) [212]. In a typical method of preparation of DdexMA (see Figure 5.85), a mixture of N,N-diethylaminoethyl dextran (Ddex),... 

FIGURE 5.85 Synthesis of methacrylated N,N-diethylaminoethyl dextran (DdexMA) (see text for description). (After liang, B., Hu, L., Gao, C, and Shen, J. 2006. Acta Biomaterialia, 2, 9. With permission.)... 

A study of the effect of diethylaminoethyl-dextran and dextran sulphate on [ S]-sulphate incorporation into glycosaminoglycans of normal and SV40 virus-transformed 3T3 cells has been reported. 

The interaction of diethylaminoethyl-dextran and bovine plasma albumin at pH values above albumin s isoelectric point has been investigated. Turbidity... 

Diethylaminoethyl Dextran (DEAD) is employed in column chromatography to separate protein molecules, due to its binding affinity to antibody or antigen bearing different net electronegative charges. 

Pagano, J.S., McCutchan, J.H., and Vaheri, A., Factors influencing the enhancement of the infectivity of poliovirus ribonucleic acid by diethylaminoethyl-dextran, /. Virol, 1,891,1967. 

Borenfreund, E., et al.(1977).Diethylaminoethyl-Dextran and uptake of nucleic acids by mammalian cells, J Nat Cancer Inst,51,1391-1392. 

Rossi, C.R. Kiesel, G.K.(1978).Bovine respiratory syncytial virus infection of bovine embryonic lung cultures enhancement of infectivity with diethylaminoethyl-dextran and virus-infected eells. Arch. Virol,5, 221-236. 

Sasaki, K. Furukawa, T. Potkin, S.A.(1981).Enhancement of infectivity of cell-free variclla zoster virus with diethylaminoethyl dextran, Proc. Soc. Exp. Biol. Med. (USA),166,281-286. 

Booth, J.C. (1977).Enhaneement of diethylaminoethyl-dextran of the plaque-forming activity of foot-and-mouth disease virus-antibody complexes in pig kidney lB-RS-2... 

Tilles, J.G. (1970).Diethylaminoethyl-dextran enhancement of interferon induction by a complex polynucleotide, Proc. Soc. Exp. Biol Med., 55, 554- 54. ... 

Wittmann, G. (1970).The use of diethylaminoethyl-dextran (DEAE-D) as adjuvant for immunization of guinea pigs with inactivated foot-and-mouth disease (FMD) virus (Article in German.), Z. Bakt.Parasit. Infektionskr. Hyg., Abt. 1. Orig,115,1-%. 

Warden, D., Thome. H.V. (1969).Influence of diethylaminoethyl-dextran on uptake and degradation of polyoma virus deoxyribonucleic acid by mouse embryo cells, J F/ra/., 4,380-387. 

This is the reason why we decided to investigate the interactions between blood elements, namely RBC and plasma proteins, and six polycations poly(L-lysine) (PLL), poly(e-lysine) (PEL), diethylaminoethyl-dextran (DEAE-dextran), poly(dimethyldiallylammonium) chloride (PDDAC), poly[2-(dimethylamino)ethyl methacrylate] (P(DMAEMA)) and Q-P(TDAE)n. 

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