Dioleoylphosphatidyl ethanolamine

Densmore, C.L., Giddings, T.H., Waldrep, J.C., Kinsey, B.M. and Knight, V. (1999) Gene transfer by guanidinium-cholesterol Dioleoylphosphatidyl-ethanolamine liposome-DNA complexes in aerosol. J. Gene. Med., 1,251-264. 

Cre CV1 DEAE-dextran DHFR DMSO DNA DOPE DOSPA recombinase (cyclization recombination) African green monkey kidney cells diethylaminoethyl-dextran dihydrofolate reductase dimethylsulf oxide deoxyribonucleic acid dioleoylphosphatidyl ethanolamine 2,3-dioleoyloxy-/V-[2(sperm inecarbox-amido)ethyl]-/V,TV-dimethyl-1-... 

Figure 19.6. A pH-sensitive lipid A-citraconyl-dioleoylphosphatidyl ethanolamine 16 and its mechanism of acid-triggered cleavage.

V-11-(2,3 Dioleyloxy)-propyl]-n,n,n-trimethylammonium chloride (DOTMA) is available in combination with the neutral helper lipid dioleoylphosphatidyl-ethanolamine (DOPE) in Lipofectin reagent (Gibco-BRL, Grand Island, NY). 

Phase Transitions in Lipid Assemblies. The rich polymorphism of amphiphilic systems, of which the multilamellar and the Hn phases are only two structures, was made evident from the seminal work of Luzzati and co-workers. Since that early work, an immense variety of water-induced phase transitions have been observed and rationalized in terms of an apparently systematic connection between water content and polar group molecular area. Therefore, the recent observation of a double transition—Hn to lamellar back to Hn—from continual hydration of dioleoylphosphatidyl-ethanolamine (40) was a surprise. Furthermore, an estimate of the cost of uncurling the monolayer in the formation of bilayers based on the previously described bending modulus far exceeds the osmotic work that actually produced the transition. Although this transition sequence can successfully be accounted for by simple thermodynamical principles, it, in fact, contains many geometry-dependent free energy contributions that we simply do not yet understand (41). 

DOTMA [2,3-bis(oleoyl)propyl]trimethyl ammonium chloride [23] DOPE dioleoylphosphatidyl ethanolamine [24]... 

Cationic liposomes are also able to interact with negatively charged cell membranes more readily than classical liposomes. Fusion between cationic vesicles and cell surfaces might result in delivery of the DNA directly across the plasma membrane. This process bypasses the endosomal-lysosomal route which leads to degradation of anionic liposome formulations. Cationic liposomes can be formed from a variety of cationic lipids, and they usually incorporate a neutral lipid such as DOPE (dioleoylphosphatidyl-ethanolamine) into the formulation in order to faciUtate membrane fiision " . A variety of cationic lipids have been developed to interact with... 

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