Dehydrogenases assay

A less time consuming alternative to the saponifica-tion/malate dehydrogenase assay is the albeit less specific measurement of the carboxylic ester groups according to Kak c and Vejdelek [10]. The sample (160 /xl) is... 

Amador, E., Reinstein, H. and Benotti, N. Precision and accuracy of lactic dehydrogenase assays. Am. J. Clin. 

Dror, Y. Sasson, H. F. Watson, J. J. and Johnson, B. C. 61ucose-6-phosphate dehydrogenase assay in liver and blood. Clin. Chim. Acta (1970), 28, 291-298. 

Standjord, P. E., and Clayson, K. J., The control of inhibitory impurities in reduced nicotinamide adenine dinucleotide in lactate dehydrogenase assays. J. Lab. Clin. Med. 67, 144 (1966). 

S.A. = 0.67 in octyl glucoside buffer) could be found in all of the detergent-extract preparations. As above, the hemoglobin step increased dramatically the S.A. of shikimate dehydrogenase assayed in detergent-extract preparations. 

The same procedure described for -hydroxysteroid dehydrogenase assay can be applied with a few differences [50]. Diaphorase (33 U/ml) is dissolved in 2 ml sodium carbonate-bicarbonate buffer (0.1 M, pH 9.0). Colour reagent is deprived of diaphorase NAD+ is replaced by NADH. The reaction is started by adding 0.1 ml diaphorase. 

Sterk JP, Stanley WC, Hoppel CL, Kemer J. A radiochemical pyruvate dehydrogenase assay activity in heart. Anal. Biochem. 

Both are abundant in skeletal muscle, myocardium, liver, and erythrocytes, so that hemolysis must be avoided, and in serum they may be assayed spectrophotometrically by their conversion of phosphate-buffered pyruvate to lactate (R6, W16) or oxalacetate to malate (S25) at the expense of added NADH2, when the rate of decrease of optical density at 340 m x thus measmes the serum activities of the respective enzymes. Recently, however, the reverse reaction has been found best for serum lactic dehydrogenase assay (A2a). In conventional spectrophotometric units the normal ranges are 100-600 units per ml for lactic dehydrogenase (W16) and 42-195 xmits per ml for malic dehydrogenase (S25) as before, one conventional spectrophotometric unit per ml = 0.48 pmoles/ minute/liter (W13). 

Chander, K. and Brooks, P.C. (1991) Is the dehydrogenase assay invalid as a method to estimate microbial activity in copper-contaminated soils Soil Biology Biochemistry, 23, 909-915. 

Turley, S. D., and Dietschy, J. M., Re-evaluation of the 3a-hydroxysteroid dehydrogenase assay for total bile acids in bile. /. Lipid Res. 19, 924-928 (1978). 

Thorpe C. (1981) Acyl-CoA dehydrogenase from pig kidney. Methods in Enzymology 71 366-74. Lehman T.C., Hale D.E., Bhala A. Thorpe C. (1990) An acyl-Coenzyme A dehydrogenase assay utilizing the ferricenium ion. Anal Biocham 186 280-4. 

Kokko L, Jaakohuhta S, Lindroos P et al (2006) Improved homogeneous proximity-based screening assay of potential inhibitors of 17 beta-hydroxysteroid dehydrogenases. Assay Drug Dev Tech 4 671-678... 

Lipoamide dehydrogenase assays LADH activity was measu-red spectrophotometrically at 340 nm using a Unicam SP 1700 instrument equipped with a SP 1805 Programm Controller and an AR 25 Linear Recorder, on liver and brain mitochondrial fractions. The assay system used was that described by Reed Willms (1966) and Ngo Barbeau (1978). ... 

The MTT ]3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and LDH (lactate dehydrogenase) assays are commonly used to evaluate the cytotoxidty of nanomaterials, while measurements of ROS, glutathione (GSH) and hOggl are used to evaluate nanomaterial-induced oxidative stress. The different cell Unes... 

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