Data independent acquisition

Data-independent acquisition Ion trap Sequential window scans 10-20 Da window (200 ms/swath) 4 (200 m/z) >2 Low-moderate... 

Several DDA add-ons and alternatives have been proposed in order to tackle the issues of poor reproducibility and proteome coverage. These studies can be grouped into two categories (i) hypothesis-driven acquisition (HDA), also called targeted acquisition and (ii) data-independent acquisition (DIA). 

Data Dependent Acquisition (DDA) and CID Fragmentation by Voltage Switching (Data Independent Acquisition, DIA)... 

Xie, H., Gilar, M. and Gebler, J. C. Characterization of protein impurities and site-specific modifications using peptide mapping with liquid chromatography and data independent acquisition mass spectrometry. Anal. Chem. 81 5699-5708, 2009. 

According to an elegant remark by Davies [5], "Modem scientific data handling is multitechnique, multisystem, and manufacturer-independent, with results being processed remotely from the measuring apparatus. Indeed, data exchange and storage are steps of the utmost importance in the data acquisition pathway. The simplest way to store data is to define some special format (i.e., collection of rules) of a flat file. Naturally, one cannot overestimate the importance of databases, which are the subject of Chapter 5 in this book. Below we discuss three simple, yet efficient, data formats. 

Multiloop Controllers The multiloop controller is a DCS network device that uses a single 32-bit microprocessor to provide control functions to many process loops. The controller operates independent of the other devices on the DCS network andTcan support From 20 to 500 loops. Data acquisition capability for up to 1000 analog and discrete I/O channels or more can also be provided by this controller. 

It has been argued that in a typical 2DLC proteomic experiment, with only a limited number of fractions submitted for analysis in the second LC dimension, chromatographic peak capacity is less than 1000. This value is considerably lower than the expected sample complexity. Additional resolution is offered by MS, which represents another separation dimension. With the peak capacity defined as the number of MS/MS scans (peptide identifications) accomplished within the LC analysis time, the MS-derived peak capacity was estimated to be in an order of tens of thousands. While the MS peak capacity is virtually independent of LC separation performance, the complexity of the sample entering the MS instrument still defines the quality of MS/MS data acquisition. The primary goal of 2DLC separation is to reduce the complexity of the sample (and concentrate it, if possible) to a level acceptable for MS/MS analysis. What is the acceptable level of complexity to maintain the reliability and the repeatability of DDA experiments remains to be seen. 

In this chapter, it was shown that filterFRET is an easy, intuitive and quantitative alternative to record sensitized emission and FRET efficiency. The major advantages of filterFRET over donor-based FRET detection methods (FLIM) are that it can be carried out with standard wide-held or confocal fluorescence microscopes that are available in most laboratories, and that it yields additional data on the acceptor population. FilterFRET is also fast, requiring just two confocal scans (if need be on a line-by-line basis) which minimizes the risk of artifacts due to, for example, organelle movement in living cells, and acquisition can be optimized for each channel independently. However, quantitative... 

The Malvern particle sizer is one of the most widely used, most effective, simple, and reliable methods commercially available for rapid measurements of ensemble characteristics of a spray. It is able to handle high droplet concentrations. It is easy to use and does not require comprehensive knowledge of its basic principles for operation. The primary advantage of the system is the speed of data acquisition and analysis. In addition, measurements of droplet size distributions can be made at any droplet velocities due to the fact that the diffraction patterns generated by droplets are independent of the... 

Data acquisition by computer refers to the use of a computer to obtain data (instrument readout values) directly by interfacing to the instrument. These data are then found in the computer s memory, or on disk, and are not necessarily recorded independently on a recorder or in a notebook. 

Local CPU and firmware for Host-independent data acquisition management... 

In contrast, NMR data collected by the States method [13] consist of real Sx) and imaginary Sy) components (fig. 6(b)) which are independently obtained during signal acquisition. The data are Fourier transformed along ti axis t2 —> F2). Then the imaginary data (7r,s) of Sx t, F2) and real data (i i,c) of Sy t, F2) are exchanged to give and of Fi)... 

SPC does require statistical quantities of product and automated data tracking. The best processes for SPC are those which are used to make large quantities of inexpensive parts. SPC is also difficult to apply to processes where the number of independent variables is large. Automated data acquisition is a must for SPC, but this is becoming inexpensive and common in the workplace. SPC is also a delayed control method. Many defective parts may be made before SPC corrects the process. The longer it takes to evaluate the results of the process, the more delay in the ability to react to process changes. Another important requirement of SPC is attention to detail on the part of the operator and/or process engineer. No battery of QC tests will detect every variation either in materials and process or in final quality. 

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