D-Gluconic acid 6-phosphate

The discovery that D-gluconic acid 6-phosphate may be degraded enzymatically to D-ribose 5-phosphate17,18 has thrown light on a possible path for the biogenesis of D-ribose. 

Several other pathways of glucose catabolism have been discovered in different microorganisms. In Pseudomonas saccharophila it was observed (100) that D-gluconic acid 6-phosphate was degraded to pyruvic acid and triose phosphate. It was postulated that an intermediate 2-keto-3-deoxy-D-gluconic acid 6-phosphate was formed. Subsequently this intermediate was isolated and characterized (101). The following pathway was suggested. 

The enthalpies for the hydrolysis of a number of glycoside and nucleoside cyclic phosphates have been measured. Photolysis of the disodium salt of a-D-glucopyranose 6-phosphate in aqueous solution furnished a number of products, including D-gluconic acid 6-phosphate, D-arabinofuranose 5-phosphate, D-glyceric acid 3-phosphate, glycolic acid phosphate, phosphoric acid, and... 

D-Gluconic acid 6-phosphate (8), (20) Purification of glycerol 3-phosphate dehydrogenase 83... 

Pyridine nucleotide-dependent dehydrogenases (C 2.1) transform aldose derivatives to uronic acids, e.g., UDP-D-glucose to UDP-D-glucuronic acid and in animals also to aldonic acids, e.g., D-glucose-6-phosphate to D-gluconic acid-6-phos-phate (glucose-6-phosphate dehydrogenase). 

D-Ribulose 5-phosphate is found together with D-ribose 5-phosphate as an oxidation product of n-gluconic acid 6-phosphate with a purified yeast enzyme (221-223). The D-ribulose 5-phosphate was identified as the o-nitrophenylhydrazone derivative, having a specific rotation in methanol of 50 5°. D-Ribulose 5-phosphate appears to be the precursor of D-ribose in yeast and rat liver. A D-ribulose diphosphate was detected in the products formed during the first few seconds of photosynthesis (224) ... 

The reaction mixture contained in a final volume of 0.1 mL, 0.1 M sodium phosphate buffer (pH 6.8), 1.0 mM tryptamine, 5 mM secologanin, and 3 mM dithiothreitol. To inhibit glucosidase activity, 100 mM D(+)-gluconic acid-5-D-gluconolactone was included. The incubation was started by addition of 10 /xL of enzyme. After 30 minutes of incubation at 30°C, the reaction was stopped by addition of 0.1 mL of 5% trichloroacetic acid. Before centrifugation, 25 /xL of 8 mM codeine hydrochloride was added as the internal standard. HPLC analysis was performed on 4 /xL aliquots. With enzyme purified to a specific activity of 710 pkat per milligram of protein, the reaction was linear with time for 1 hour and with protein up to at least 50 /xg of protein during a 20-minute incubation period. 

Dextroi OC-15. See Nonoxynol-6 phosphate Dextroi OC-22. See Nonoxynol-9 phosphate Dextroi OC-40. See Trideceth-3 phosphate Dextroi OC-60. See Trideceth-6 phosphate Dextroi OC-70. See Trideceth-10 phosphate Dextroi Defoamer AL-7. See Silicone Dextro-limonene. See d-Limonene Dextronic acid. See D-Gluconic acid Dextropimaric acid. See Pimaric acid Dextrose a-Dextrose. See Glucose Dextrose monohydrate. See D-Glucose monohydrate Dextrosol. See Glucose Dexulate. See Dextran sulfate sodium DEZ DEZn. See Diethyl zinc DeZOLINE T. See Tall oil hydroxyethyl, imidazoline... 

C.z.e. at pH 12 with pulsed amperometric detection permitted glucose in blood to be determined at Iff M levels, and the separation of 2 amino 2-deoxy-D-glucose, its 6-phosphate and 6-sulfate, and 2-amino-2-deoxy-D-gluconic acid. ... 

Phospho-D-gluconic acid is formed from gluconic add by yeast glucono-kinase and from glucose 6-phosphate by the corresponding dehydrogenase or by bromine oxidation ... 

The 2-keto-3-deoxy-aldonic acid (phosphate) aldolases from Pseudomonas strains - 3-deoxy-2-keto-L-arabonate (F.C 4.1.2.18), 3-deoxy-2-keto-D-xylonate (EC 4.1.2.28), 3-deoxy-2-keto-6-phospho-D-gluconate (EC 4.1.2.14) and 3-deoxy-2-keto-6-phospho-D-galactonate aldolase (EC 4.1.2.21) - appear to be specific even for the acceptor components, but allow stereoselective syntheses of the respective natural substrates29. 

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