Culture of NRRL

A culture of NRRL 2773 is produced by growing the organism ing the following composition on a nutrient agar slant hav-... 

A lyophilized culture of Streptomyces spectabih s, NRRL 2792, was used to seed the follow-... 

Isolation from cultures of Eupenicillium hrefeldianum NRRL 5734. a Amberlite IRA-411/pH 10. b Chromatography on DEAE-Sephadex A-25. 

Bluensomycin was obtained from cultures of Streptomyces verticillus, or the same substance produced by any other means. For example antibiotic was prepared by growing of Streptomyces bluensis NRRL 2876 biological way and isolation from cultural solution by adsorption with a cation-exchange resin or a capillary adsorption method by elution with water-acid solution at pH from 1 to 6 or acidic water solution of acetone. 

The process for producing spiramycin comprises inoculating an aqueous nutrient medium with a culture of the NRRL No. 2420, allowing aerobic fermentation to take place and separating from the culture medium the spiramycin thus formed. The culture medium also contains the antibiotic substance known as Congocidin which, however, does not possess the same useful properties as spiramycin and which can be isolated in crystalline form. The separation of the two antibiotic substances is readily achieved. 

The isolation and culture of Claviceps paspali Stevens et Hall NRRL 3080 may be carried out, for example, in the following manner ... 

Actinomycin F, Actinomycin KS4 KS4. An antibiotic produced by cultures of actinomycin C-elaborat-ing strains of Streptomyces, such as Streptomyces BOP 476 (NRRL 2580) and Sirt-piomy r. chrysomaltus (NRRL 2250). Considered to be a derivative of actinomycin C2 amino acid analysis indicates that the prolines present in actinomycin C2 are substituted by sarcosine in actinomycin I, Production by controlled biosynthesis Schmidt-Kastner. Naturwiss. 43, 131 (1956) idem, Ann. N.Y. Acad. Set. 89, 199 (1960) Schmidt-Kastner, Hackmann, U.S. pat. 3,219,544 (1965 to Bayer). 

At the other extreme, Borghi et al. reported the biotransformation of the teicoplanin family of antibiotics. They found that these large, complex glyco-peptides produced by Actinoplanes teichomyceticus could be demannosylated by cultures of Nocardia orientalis NRRL 2450, or Streptomyces candidus NRRL 3218. Interestingly, they also found that the demannosylated teicoplanin and other de-mannosylated derivatives could be converted back to the mannosylated form by the original producing organism (31). 

The avermectins are a group of closely related 16-membered macrocyclic lactones extracted from a fermentation broth produced by a culture of Strepto-myces avermitilis MA-4680 (NRRL 8165) isolated at the Kitasato Institute from a soil sample collected at Kawana, Ino City, Shizuoka Prefecture, Japan [4]. They are the most potent anthelmintic and acaricidal compounds known. Avermectin structures are shown in Fig. 1. 

Plattner RD, Tjarks LW, Beremand MN (1989) Trichothecenes Accumulated in Liquid Culture of a Mutant of Fusarium sporotrichioides NRRL 3299. Appl Environ Microbiol 55 2190... 

Bergers WWA, van der Stap JGMM, Kientz CE (1985) Trichothecene Production in Liquid Stationary Cultures of Fusarium tricinctum NRRL 3299 (Synonym F. sporotrichioides) Comparison of Quantitative Brine Shrimp Assay with Physicochemical Analysis. Appl Environ Microbiol 50 656... 

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