Lyophilization cultures

Germination Stage A lyophilized culture of M. purpurea is added to a 300 ml shake flask... 

Tank fermentation of Micromonospora inyoensis — Germination stage 1 Under aseptic conditions, add a lyophilized culture (or cells obtained from a slant culture) of M. inyoensis to a 300 ml shake flask containing 100 ml of the following sterile medium ... 

A lyophilized culture of Streptomyces spectabih s, NRRL 2792, was used to seed the follow-... 

Figure 4. Preparative isoelectric focusing in a granulated gel of a desalted lyophilized culture filtrate (50 mg protein) from the late exponential growth phase of Thermoactinomyces sp. Separation carried out for 48 hr at a constant power of 8 W (29). (C>) CM-cellulase activity (A) Avicelase activity (6).

In operations similar to the E. ashbjii procedures, the closely related fungus A.shbjagossjpii gave similar yields. Thus, a yield of 7.3 g/L was obtained with a lyophilized culture in a medium containing fat, leather glue, and com extracts (50), and 6.420 g/L with bone or hide fat, alone or in a mixture with other plant or animal fats as the carbon source (51). The yield from immersed cultures of. gossjpii was increased to 6.93—7.20 g/L by use of waste fats or technical cod-hver oil (52). 

FIG. 5 Viability of Saccharomyces cerevisiae (o) and Oenococcus oeni strain EQ-54 ( ) inoculated into a Chardonnay juice with the bacteria prepared using a diluted grape juice medium (A) or a lyophilized culture (B). (Adapted from Semon et al, 2001 and with the permission of the Australian Journal of Grape and Wine Research.)... 

Figure 7. Comparison of 20 serum samples assayed by the RMA using standard and lyophilized cultures of L. leichmannii correlation coefficient, r = 0.98. Earn point represents mean of duplicate determinations. Line of identity is drawn in.

There is some evidence to suggest that soluble compounds produced by lactic acid bacteria may act directly on tumor cells to inhibit their growth. Arimochi et al. (1997) showed (a) an inhibitory effect of L. acidophilus on azoxymethane-induced ACF formation in rat colon and (b) enhanced removal of 0 -methylguanine from the colonic mucosal DNA. This inhibitory effect was found in culture supernatants and not from bacterial cells. Furthermore, other studies have shown that dietary administration of lyophilized cultures of B. longum suppressed azoxymethane-induced colonic tumor development, along with a decrease in colonic mucosal cell proliferation and colonic mucosal and tumor ornithine decarboxylase and ras-p21 activities (Reddy, 1998). 

Duke, G.R. 1979. Factors influencing the survival and utilization of lyophilized cultures of Leuconostoc oenos PSU-1 for inoculation of wines. Master of Science Thesis. The Pennsylvania State University, Department of Food Science,... 

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