Cultural strategies

A comparison between insect and mammalian cell culture technology shows various similarities and differences. Due to the fact that insect cell lines typically derive from specific organs or non-differentiated embryonic tissues, the same cell line can proliferate in suspension or in adherent manner (in monolayer). This versatility in growth mode, which contrasts with most mammalian cell lines, increases the choices of bioreactor types and culture strategies for production. Contact inhibition shown by various mammalian cell lines is low or absent for insect cells, and they tend to aggregate in suspension or in adherent cultures. 

Arden N, Betenbaugh MJ (2004), Life and death in mammalian cell culture strategies for apoptosis inhibition, Trends Biotechnol. 22 174-180. 

The use of plant suspension cells for the production of biopharmaceutical proteins has many advantages, including safety, defined growth conditions, containment, and the ability to use continuous-culture strategies. However, some challenges remain to... 

Organizational issues include the management of human resources, financial allocations, equipment and facilities. Organizational process contains safety culture strategy, procedures and safety policy in relation to economic profit. 

The heterologous expression of metabolically related enzymes represents one way to overcome several of the limitations and drawbacks of the above-described mixed culture strategies. Within a biocatalytic context, such an approach is appeahng only when all enzymes involved display sufHdently broad substrate tolerance in order to ultimately form a system capable of converting various starting materials. 

Azhar EA, Hemmat M.Abdelhady AM, Abdel H, KhodairTA. Batch production of polyhydroxybutyrate (PHB) by Ralstonia eutropha and Alcaligenes latus using bioreactor different culture strategies. J Appf Sci Res 2009 5 556-64. 

Duarte, A. R. C., Mano, J. F. and Reis, R. L. (2011) Thermosensitive polymeric matrices for three-dimensional cell culture strategies. Acta Biomaterialia, 7, 526-529. 

Several 3D culture strategies for hPSCs have been established (a) cell culture in macroporous scaffolds, (b) cell culture on nanofibers or microfibers, (c) self-aggregated spheroid (cell aggregate) culture, (d) cell culture on microcarriers, and (e) microencapsulated cell culture in suspended hydrogels [97,98]. 

Bioreactor Configuration Cell Type Culture Strategy References... 

Garcia Canclini, N. (1989). Hybrid Cultures Strategies for Entering and Leaving Modernity. Minneapolis, MN University of Minnesota Press. 

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