Cell culture process development strategy based

However, liquid-liquid extraction can form emulsions, and the extractant is usually expensive and sometimes toxic to the cell culture (Diirre, 1998). To solve these problems, the perstraction technique was developed based on the liquid-liquid extraction concept. In the perstraction separation process, the fermentation broth and the extractant are separated by a membrane, where two immiscible phases can exchange butanol. In this process, butanol can diffuse preferentially across the membrane, while other components (substrates, cell culture, and other fermentation products) are retained in the fermentation broth. This strategy effectively avoids the potential problems in the liquid-liquid extraction system, but it needs to point out that... 

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