Cross-linked polyacrylamide gels preparation

Preparation of Ethylene Diacrylate Cross-Linked Polyacrylamide Gel ... 

This resin has been shown to be specific to Fe + ions. In an alternative technique [29], cross-linked polyacrylamide is prepared by maintaining a solution of acrylamide, A/, A/ -methylenebisacrylamide with ammonium persulfate at 25°C. A solution of hydroxylamine hydrochloride is added to the gel, and the pH of the reaction mass is raised to 12 by adding sodium hydroxide. The reaction is carried out for 24 h, and ammonia is released as the hydroxamic acid groups are formed on the matrix of the gel. The polyacrylamide gel P—CO—NH2 is modified through the following mechanism ... 

Cross-linked polyacrylamides attached with morpholine pendent at some repeating unit of the backbone chains have been prepared and used for the separation of discrete chemical compounds by gel-permeation chromatography (83). 

Premixed blends of acrylamide and bisacrylamide prepared with varius ratios of monomers were purchased from Eastman Kodak Chemical Company (Rochester, NY). The 37.5 1 and the 19 1 preparations were used for the study. Gels made from these mixtures will be referred to as 2.6% and 5% cross-linked polyacrylamides, respectively. Five grams of each monomer blend were added to 95-g portions of distilled water. Solution was achieved by mixing for 1 h. To each sample was added 1 ml each of a 1% solution of N, N, N, N -tetramethylethylenediamine (Eastman Chemical Co., New Haven, CT) and a 10% solution of ammonium persulfate (Mallinckrodt Laboratory Chemicals, Phillipsburg, N.1). The solutions were poured into an open polyethylene mold and allowed to cure for 12 h at room temperature. The gels were carefully removed and placed in an excess of distilled sterile water for 48 h. The water was replaced several times during the equilibration period. It was felt that this was sufficient to remove unreacted monomers and impurities. The gels were then cut with a steel-ruled die into circles 40 imn in diameter. 

Uses organic intermediate cross-linking agent for preparation of polyacrylamides in preparing poly-acrylamide gel for electrophoresis, chemical grouting and other purposes in NAPP and Nyloprint UV-cured printing plates A... 

Polyacrylamide gels of the two cross-linking levels mentioned above were prepared and cut by the same procedure. The circles were placed in a IN sodium hydroxide solution. Conversion of the amide groups to the carbonyl was evidenced by the... 

This so-called phosphodiester method [92] was later also performed on cross-linked polystyrene [93], macroporous 25% cross-linked polystyrene [94], silica [95], poly(-vinyl alcohol) [96,97], proteins [98], PEG [99], and polyacrylamide [100]. This extensive evaluation of different supports stemmed from the observation that cross-linked polystyrene or other gel-type supports were not ideal for the preparation of phospho-... 

Polyacrylamide is a cross-linked polymer of acrylamide. These gels are more difficult to prepare than agarose. Monomeric acrylamide (which is a known neurotoxin) is polymerized in the presence of free radicals to form polyacrylamide. The free radicals are provided by ammonium persulfate and stabilized by TEMED (/V/V/V/V -tetramethylethylenediamine). The chains of polyacrylamide are cross-linked by the addition of methylene-bisacrylamide to form a gel whose porosity is determined by the length of chains and the degree of cross-linking. The chain length is proportional to the acrylamide concentration usually between 3.5 and 20%. Cross-linking bis-acrylamide is usually added at the ratio 2 g bis/38 g acrylamide. 

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