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Cre-recombinase

Cre is a bacterial recombinase (cre=causes recombination), which recognizes loxP sites of bacteriophage P. If two loxP (loxP= locus of x-ing over of bacteriophage P) sites have a parallel orientation, the DNA segment between these sites will be deleted by the action of the Cre recombinase. [Pg.396]

Marker excision by site-specific recombination Very clean excision, small footprint Complex cloning procedure. Requires additional transgene encoding Cre recombinase 39... [Pg.257]

P = constitutive promoter, tetR = TETrepressor gene,TETR = TET repressor protein, tetO = TET operator sequence, = tetracycline, ere/CRE = Cre recombinase gene/protein, PLEA = late embryogenesis abundant promoter, RIP = gene for ribosome inactivating protein, shaded blocks are loxP sites in orientation shown by solid triangle. After [48]... [Pg.261]

A widely used inducible system in the mouse is the bacteriophage Pl-derived Cre-LoxP system (25). The site-specific Cre recombinase recognizes specifically 34 bp loxP sites. Generating mice that harbor a DNA sequence flanked by two loxP sites and... [Pg.286]

Sauer B, Henderson N (1988) Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage PI. Proc Natl Acad Sci USA 85 5166-5170... [Pg.303]

Key words RNAi, Conditional, ShRNA, Cre recombinase, TetR/O, Transgenic mice, Rosa26,... [Pg.307]

Nagy, A. (2000) Cre recombinase the universal reagent for genome tailoring. Genesis 26, 99-109. [Pg.75]

Danielian, P.S., Muccino, D., Rowitch, D.H., Michael, S.K., and MacMahon, A.P. (1998) Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase. Curr. Biol. 8, 1323-1326. [Pg.75]

Feil, R., Wagner, J., Metzger, D., and Chambon, P. (1997) Regulation of Cre recombinase activity by mutated estrogen receptor ligand-binding domains. Biochem. Biophys. Res. Commun. 237, 752-757. [Pg.75]

Zhang, Z. and Lutz, B. (2002) Cre recombinase-mediated inversion using lox66 and lox71 method to introduce conditional point mutations into the CREB-binding protein. Nucl. Acids Res. 30, e90. [Pg.75]

Tiscornia, G., Tergaonkar, V., Galimi, F., and Verma, I.M. (2004) Cre recombinase-inducible RNA interference mediated by lentiviral vectors. Proc. Natl. Acad. Sci. USA 101, 7347-7351. [Pg.78]

See other pages where Cre-recombinase is mentioned: [Pg.87]    [Pg.89]    [Pg.258]    [Pg.260]    [Pg.866]    [Pg.866]    [Pg.228]    [Pg.228]    [Pg.228]    [Pg.239]    [Pg.240]    [Pg.285]    [Pg.287]    [Pg.303]    [Pg.307]    [Pg.311]    [Pg.322]    [Pg.418]    [Pg.36]    [Pg.154]    [Pg.986]    [Pg.1501]    [Pg.1570]    [Pg.1572]    [Pg.1804]    [Pg.455]    [Pg.455]    [Pg.477]    [Pg.40]    [Pg.62]    [Pg.242]    [Pg.243]    [Pg.244]    [Pg.245]    [Pg.417]   
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Recombinases

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