Marker excision

Marker excision by site-specific recombination Very clean excision, small footprint Complex cloning procedure. Requires additional transgene encoding Cre recombinase 39... 

Marker excision using the X attB system Very clean excision, small footprint. Spontaneous excision Efficiency 40... 

Myronovskyi, M., Rosenkranzer, B., and Luzhetskyy, A. (2014) Iterative marker excision system. Appl Microbiol Biotechnol, 98 (10), 4557 -4570. 

Because of the possible effects of active and carrier-mediated processes and metabolic biotransformation, the issue of tissue viability is important for in vitro buccal mucosal experiments. The barrier nature of the buccal mucosa resides in the upper layers of the epithelium, where unlike in the stratum corneum, the cells contain a variety of functional organelles [119, 122, 125, 150], and so tissue viability may be an important component of the barrier function of the tissue. Various methods have been employed to assess the viability of excised buccal mucosa, including measurement of biochemical markers, microscopic methods, and linearity of transport data [42], While biochemical methods, including measurement of adenosine 5 -triphosphate (ATP) levels and utilization of glucose, provide information on the metabolic activity of the tissue, this does not necessarily relate to the barrier function of the tissue. In excised rabbit buccal mucosa, levels of ATP were measured and found to decline by 40% in 6 h, and this correlated well with transmission electron microscopic evaluation of the tissue (intact superficial cells) [32], In addition, the permeability of a model peptide was unaltered up to 6 h postmortem, but at 8 h, a significant change in permeability was observed [32], These investigators therefore claimed that excised rabbit buccal mucosa could be used for diffusion studies for 6 h. 

Goldsbrough, A.P., Tong, Y., and Yodder, J.I., Lc as a non-destructive visual reporter and transposon excision marker gene for tomato. Plant J., 9, 927, 1996. 

Neumann AS, Sturgis EM, Wei Q. Nucleotide excision repair as a marker for suseeptibility to tobaeeo-related cancers a review of molecular epidemiological studies. Mol Carcinog 2005 42 65-92. 

The product yields in y-irradiated DNA are given in Tables 12.5-12.7. FAPY-G has always been observed, but 8-oxo-G yields were extremely low, when y-ir-radiations were carried out under N2. This may serve as a caveat for the common practice to use 8-oxo-G as a kind of marker for free-radical DNA damage (for assays see Chap. 13.2). Besides the other products reported in Table 12.5, 8 cG lesions per 106 bases are formed per Gy in y-irradiated N20-saturated DNA (50 pg ml-1) solution (Dizdaroglu et al. 2001a corresponding to 1.6 x 10 9 mol J-1), and this lesion has also been observed in y-irradiated cultured human cells (Dizdaroglu 1986 for its elimination by nucleotide excision repair see Kuraoka et al. 2000). 

In Part 1 [1] we described a protocol for the evaluation of measurement uncertainty from validation studies such as precision, trueness and ruggedness testing. In this paper we illustrate the application of the protocol to a method developed for the determination of the dyes Cl solvent red 24 and Cl solvent yellow 124, and the chemical marker quinizarin (1,4-dihydroxyanthra-quinone) in road fuel. The analysis of road fuel samples suspected of containing rebated kerosene or rebated gas oil is required as the use of rebated fuels as road fuels or extenders to road fuels is illegal. To prevent illegal use of rebated fuels, HM Customs and Excise require them to be marked. This is achieved by adding solvent red 24, solvent yellow 124 and quinizarin to the fuel. A method for the quantitation of the markers was developed in this laboratory [2]. Over a period of time the method had been adapted to improve its performance and now required re-validation and an uncertainty estimate. This paper describes the experiments under-... 

Nodular lymphoid hyperplasia BALT has replaced the term pseudolymphoma and is characterized by reactive lymphoid proliferation that characteristically shows numerous lymphoid follicles with large germinal centers usually occurring in middle-aged people, most of whom are asymptomatic. Approximately 10% to 15% of patients have a collagen vascular disease such as systemic lupus erythematosus or an immune disease of uncertain etiology, and they frequently exhibit polyclonal gam-mopathy. Polytypic plasma cells are common. Marker studies show a mixed population of CD4- and CD8-positive T cells. Most cases occur as solitary nodules and reoccur in up to 15% of surgically excised cases. 

---