Cre-loxP system

Flp/FRT is a system analogous to the cre/loxP system. Flp is an yeast enzyme that recognizes FRT sites. If two FRT sites have a parallel orientation, the DNA segment between these sites will be deleted by the action of the Flp recombinase. 

A widely used inducible system in the mouse is the bacteriophage Pl-derived Cre-LoxP system (25). The site-specific Cre recombinase recognizes specifically 34 bp loxP sites. Generating mice that harbor a DNA sequence flanked by two loxP sites and... 

Analogously to the Cre-LoxP system, the 34 bp Flippase recognition target (FRT) sites are recognized by the Flippase recombination enzyme (FLP) derived from Saccharomyccs cerevisiae (28). The FLP-FRT recombination system is also utilized for the inducible genetic manipulation of the mouse genome (29, 30). 

Since the reaction doesn t require accessory protein factors and can be performed in vitro with a variety of DNA substrates, the Cre-loxP system is much used in genetic engineering.583 584 A pair of related integrase subunits known as XerC and XerD perform a similar function for the E. coli chromosome as well as for multicopy plasmids.585 586 The XerC / XerD system is... 

Nagayama Y, Nishihara E, litaka M, Namba H, Yamashits S, Niwa M. Enhanced efficacy of transcriptionally targeted suicide gene/prodnig therapy for thyroid carcinoma with the Cre-loxP system. Cancer Res 1999 59 3049-3052. 

Sakai Y, Kaneko S, Sato Y, Kanegae Y, Tamaoki T, Saito I, Kobayashi K. Gene therapy for hepatocellular carcinoma using two recombinant adenovirus vectors with alpha-fetoprotein and cre-loxP system. J Virol Methods 2001 92 5-17. 

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