Cre/loxP recombination

Kndoh, H., Ikeda, H., Kakitani, M., Ueda, A., Hayasaka, M., Tomizuka, K., et al. (2005) A new model mouse for Duchenne muscular dystrophy produced by 2.4 Mb deletion of dystrophin gene using Cre-loxP recombination system. Biochem Biophys Res Commun 328, 507-516. 

Kuhn, R. and R. Torres (2002) Cre/loxP recombination and gene targeting. Methods in Molecular Biology 180,175-204. 

A disadvantage of these systems is probably the lower efficiency and convenience of cloning libraries of DNA molecules. This difficulty has been alleviated by using a Cre-loxP recombination strategy the fusion gene is cloned in a small plasmid and then efficiently recombined in vivo into the phage [25],... 

Disruption of the STAT3 gene in neutrophils and macrophages) by Cre/loxP recombination Enhanced IL-7 production by mucosal T cells STAT4 overexpression... 

Smith, A. J. H., Sousa, M. A. D., Kwabi-Addo, B., Heppell-Parton, A., Impey, H., and Rabbitts, P. (1995) A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nature Genetics 9,376-385. 

Kasim V, Miyagishi M, Taira K (2003) Control of siRNA expression utilizing Cre-loxP recombination system. Nucleic Acids Res Suppl 255-256. 

Cre is a bacterial recombinase (cre=causes recombination), which recognizes loxP sites of bacteriophage P. If two loxP (loxP= locus of x-ing over of bacteriophage P) sites have a parallel orientation, the DNA segment between these sites will be deleted by the action of the Cre recombinase. 

Muller W, Testa G, Roers A Mast cell-specific Cre/ loxP-mediated recombination in vivo. Transgenic Res 2008 17 307-315. 30... 

Figure 4.3. Univector plasmid fusion system. Cre- loxP mediated site-specific recombination fuses the pUNI and pHOST plasmids at the loxP site. As a result, the gene of interest is placed under the control of the pHOST promoter and fused to any Tag sequences present in the pHOST plasmid.

Gopaul, D. N., Guo, F. and Van Duyne, G. D. (1998). Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination. EMBO J. 17, 4175-4187. 

Analogously to the Cre-LoxP system, the 34 bp Flippase recognition target (FRT) sites are recognized by the Flippase recombination enzyme (FLP) derived from Saccharomyccs cerevisiae (28). The FLP-FRT recombination system is also utilized for the inducible genetic manipulation of the mouse genome (29, 30). 

Van Duyne, G.D. (2001) A structural view of Cre-loxP site-specific recombination. Annu. Rev. Biophys. Biomol Struct. 30, 87-104. 

Other research groups are combining stress inducible promoters with the Cre-LoxP site specific recombination system of PI bacteriophage. Scott et al. (2000) have combined the ionizing radiation inducible EGR-1 promoter with this recombination system to express herpes simplex vims thymidine kinase (HSV-tk). With this combination system, radiation doses relevant for cancer treatment can be used to... 

Sauer, B. and Henderson, N. (1989) Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome. Nucl. Acids Res. 17, 147-161. 

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