Liposome conjugation

Anabousi S, Bakowsky U, Schneider M, Huwer H, Lehr CM, Ehrhardt C (2006) In vitro assessment of transferrin-conjugated liposomes as drug delivery systems for inhalation therapy of lung cancer. Eur J Pharm Sci 29(5) 367-374... 

M. J. Turk, D. J. Waters, and P. S. Low. Folate-conjugated liposomes preferentially target macrophages associated with ovarian carcinoma. Cancer Lett. 213 165-172 (2004). 

Bohl-Kullberg E, et al. Development of EGF-conjugated liposomes for targeted delivery of boronated DNA-binding agents. Bioconjugate Chem 2002 13 737. 

Konigsberg, P.J., R. Godtel, T. Kissel, and L.L. Richer, The development ofIL-2 conjugated liposomes for therapeutic purposes. Biochim Biophys Acta, 1998. 1370(2) 243-51. 

Reddy, J.A., Dean, D., Kennedy, M.D. and Low, P.S. (1999) Optimization of folate-conjugated liposomal vectors for folate receptor-mediated gene therapy. J. Pharm. Sci., 88, 1112-1118. 

Gosk, S., et al. 2004. Targeting anti-transferrin receptor antibody (0X26) and OX26-conjugated liposomes to brain capillary endothelial cells using in situ perfusion. J Cereb Blood Flow Metab 24 1193. 

Fujiwara, M., J.D. Baldeschwieler, and R.H. Grubbs. 1996. Receptor-mediated endocytosis of poly(acrylic acid)-conjugated liposomes by macrophages. Biochim Biophys Acta 1278 59. 

Aliquots of conjugated liposomes (about 0.4 pmol lipid) were first dried under vacuum using a Speed Vac. 

Fig. 3. Competitive Immunoassay (A) In the absence of analyte, analyte-conjugated liposomes are captured by an analyte-specific antibody at the competition zone. (B) In the presence of analyte, analyte-tagged liposomes compete with analyte present in the sample for the antibody immobilized at the competition zone yielding a signal that is inversely proportional to the analyte concentration.

Add 4 pL hybridization buffer, 1 pL NASBA product, and 2 pL reporter probe conjugated liposomes to the bottom of a 10 x 75 mm culture tube. 

Shmeeda, H., Mak, L., Tzemach, D., et al. 2006. Intracellular uptake and intracavitary targeting of folate-conjugated liposomes in a mouse lymphoma model with up-regulated folate receptors. AfoZ Cancer Ther. 5 818-24. 

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