Commercial Lateral-Flow Immunoassays for Milk, Animal Tissues, and Honey

3 Commercial Lateral-Flow Immunoassays for Milk, Animal Tissues, and Honey 

A wide variety of commercial LFIA kits for the detection of antibiotic residues is available and the most well-characterised of these are summarized in Table 5.4, including the rapid one-step assay (ROSA) range from Charm Sciences Inc., and the Tetrasensor, Twinsensor, Trisensor, and Sulfasensor from Unisensor SA and the Betastar from Neogen Corporation. Other LFIA assays have been reported in the scientific literature for the detection of antimicrobial residues, including a lateral-flow device for nicarbazin detection in animal feedstuffs. However, at present these are not commercially available. These LFIA tests incorporate either a receptor protein or an antibody as the specific capture molecule and operate in the competitive assay format (most applicable for small-molecule detection). The sample preparation protocols are based on either direct analysis of the liquid sample (e.g., milk) or a simple extraction step for solid or complex matrices using buffer(s) supplied in the test kit. In general, the time required to perform these tests is less than 30 min with only basic laboratory equipment, if any, required. 

TABLE 5.4 Summary of Commercially Available Lateral-Flow Immunoassays for Detection of 

Testkit Manufacturer and Website Test Name Class and Number of Target Analyte(s) Application(s) Test Time (mins) 

Unisensor SA Tetrasensor— animal Tetracyclines (5) urine, and fish Tissues, egg, fish. 15 

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