Colpoda

The technology of dry culture Colpoda steinii production is developed adjusted in the Ukraine (the Veterinary Products Company Renaissanc M ). 

We studed a toxicity of some medicinal preparations on culture of the primerely trepsinised chicken fibroblasts (TCF) and biotesting with use of Colpoda steinii infusorians Results of researches are submitted in the table 1. 

Table 1. Results of a toxicity definition of some medicinal preparations on Colpoda steinii and TCF.

Ethonium and Decametaximun have shown rather high toxicity at both methods of testing whereas Zovirax (Acyclovir), E-aminocapronic acid, Ambenum and Sodium Aceminatum had a hypo toxicity at their influence on cells monolayer, and Colpoda steinii infusorians culture. 

Thus, from the moment of the beginning for biotesting with Colpoda steinii preparation and results reception passed 16 - 19 hours, this work did not need a sterile conditions and was carried out by one employee. 

Vinokhodov D.O., Vinokhodov V.O. Colpoda steinii as a test organism / Infusoria in bioassays, Saint Petersburg, 1998.PP.85-87. 

As protozoa and nematodes live in pore water in the soil, most of the methods are adapted from toxicity tests designed for aquatic samples. Among the protozoa the tests with cihates Tetrahymena pyriformis, Tetrahymena thermophiia, Colpoda cucullus, Colpoda inflata, Colpoda steinii, Paramecium caudatum, and Paramecium aurelia have been developed [ 102,112-117]. It is the opinion of some authors that the sensitivity of infusorians is higher than that of microorganisms [115,116]. 

Campbell, C. Warren, A. Cameron, C. Hope, S. Direct toxicity assessment of two soils amended with sewage sludge contaminated with heavy metals using protozoan Colpoda steinii) bioassay. Chemosphere 1997, 34, 501-514. 

Forge, T.A., M.L. Berrow, J.J. Darbyshire, and A. Warren. 1993. Protozoan bioassays of soil amended with sewage sludge and heavy metals, using the common soil ciliate Colpoda steinii. Biol. Fertil. Soils 16 282-286. 

Kassab K, Ben Amor T, Jori G, Coppellotti O. Photosensitisation of Colpoda inflata by meso-substituted cationic porphyrins. Photochem Photobiol Sci 2002 1 560-4. 

Some of the most widespread ciliates, e.g. Glaucoma scintillans, Colpidium colpoda and species of the Paramecium aurelia and the Tetrahymena pyriformis-complex very likely lack the ability to produce resting cysts, although they have... 

Meier-Tackmann, D., Wenzel, F. (1988). Daten iiber Wettergeschehen und Reaktivierbarkeit der Dauercysten von Colpoda cucullus O.F. Muller (Holotricha, Ciliata). Zoologischer Anzeiger 220, 277-290. 

The only significant doublets are those of microorganisms, for example Colpidium colpoda with Beggiatoa Nitzschia palea with Stigeoclonium Paramecium trichium with Navicula accommoda. 

Protozoa (Colpoda mauposi) Freshwater DEV LIO Designed for wastewater... 

Colpoda The problem with Colpoda (especially C. steinii) appears similar to that with Paramecium. No attempts have been made, however, to substitute for the plazmoptyzate medium the medium of Johnson which was successful on two species of Paramecium. An obvious advantage of Colpoda is its high reproduction rate and its high maximum yield. 

Certain Berberis aUcaloidal salts, particularly berberine, oxyacanthine, and columba-mine, have bactericidal activities. In one study, berberine chloride showed higher activity than chloramphenicol against Staphylococcus epidermidis, Neisseria meningitidis, Escherichia coli, and other bacteria. Oxyacanthine chloride at 0.01% and columbamine chloride at 1.0% killed Bacillus subtilis and Colpidium colpoda ... 

Burbank, W. D. Physiology of the ciliate Colpidium colpoda. I. The effect of various bacteria as food on the division rate of Colpidium colpoda. Physiol. Z06I. 15, 342 (1942). 

Kidder, G. W., and C. A. Stuart Growth studies on ciliates. I. The role of bacteria in the growth and reproduction of Colpoda. Physiol. Z06I. 12, 329 (1939). 

---