Collagen isolation

Swatschek, D., Schatton, W., Kellermann, J., Mullaer, W. E. G., and Kreuter, J. (2002). Marine sponge collagen Isolation, characterization and effects on the skin parameters surface-pH, moisture and sebum. Eur. ]. Pharm. Biopharm. 53,107-113. 

Table I. Mole Ratio of Proline to Hydroxyproline in Collagen Isolated from Granuloma of Normal and Scorbutic Guinea Pigs0...

The origin and the characteristic properties of the initial material, and The method of collagen isolation and processing... 

Swasdison, S., Mayne, P.M, Wright, D.W. et al (1992). Monoclonal antibodies that distinguish avian type I and type III collagens isolation, characterization and immunolocalization in various tissues. Matrix, 11, 56-65. 

Hydration Effects on Protein Dynamics. Solid state NMR investigations of the effect of temperature and hydration on the molecular mobility of collagen isolated from bovine achilles tendon have been reported. " C CP MAS experiments were performed on samples at natural abundance using NMR methods... 

Kimura, S., and Tanzer, M. L., 1977, Nereis cuticle collagen Isolation and characterization of two distinct subunits, Bioch., 16 2554. 

Collagen is also available commercially (e.g., Vitrogen). However, the gelling properties of collagen isolated from rat tails are somewhat better than those of commercially obtained samples. Furthermore, the currently tested whole-mount in-sim hybridization techniques do not appear to work well on commercially obtained collagen. 

Chemical and physical analyses of the collagen isolated from cell lines derived from a mouse teratocarcinoma source or a mouse blastocyst source have identified a type V collagen, with distinct differences from types I, II, HI, and IV collagen. ... 

The carbohydrate content of collagens isolated from patients with idiopathic pulmonary fibrosis appears to be normal. ... 

Gelatin is prepared by the thermal denaturation of collagen, isolated from animal and fish skin and bones, with very dilute acid. Gelatin has also been successfully and largely used as a matrix to produce bionanocomposites which are reinforced with nanoreinforcements such as clays and hydroxyapatite to be used in applications ranging from packaging materials to artificial bones. 

Ogawa, M., Portier, R.J., Moody, M.W., Bell, J., Schexnayder, M.A., and Losso, J.N. (2004) Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum Pogonia cromis) and sheepshead seabream Ardwsargus probatocephalus). Food Chem., 88. 495-501. 

---