Coated rod TLC-FID systems

In the coated rod TLC-FID systems, the components of various chromatographic fractions are vaporized in the flame jet partly by physical evaporation and partly by pyrolysis, i.e. breakdown of... 

The coated rod TLC-FID system using Chromarods and the latroscan TH-IO instrument have found numerous applications for a wide variety of substances. Table 2 lists some of the applications of this coated rod TLC-FID system from readily accessible literature. Further applications are detailed in earlier reviews (16,17,17a,26a-26e) and the brochures provided by latron Laboratories, Inc. 

Some recent developments involving the use of novel vapor phase detectors, such as the flame thermionic ionization detector (FTID), which responds to compounds containing nitrogen and halogen atoms, and the flame emission photometric detector (33), which detects substances containing sulfur and/or phosphorus as well as chemiluminescent nitrogen detector, coupled on-line with FID (33a), should be able to widen the range of possible applications of the coated rod TLC-FID systems. 

Flame ionization detectors (FID), 171,361-372 applications of TLC-FID systems, 369 coated rod systems, 366-369 tubular systems, 365 TLC-FID systems, 362-369 coated rod systems, 364-369 tubular systems, 362-364 Flame diermionic ionization detector (flll>), 369 Flat-bed chromatography, 3 Flavonoids, 717-722 distribution, 717 practical experiments, 719-722 structure, 717 TLC of, 718-719 Fluorescence, 939 measurement of, 208-210 for quantitative determination in TLC, 281-287 Fluorgenic detection Q>esticide detection system), 810-811 Folic acid, 1051 Food additives, 803 Food dyes, 1006-1014 Foodstuff dyes, 1004... 

A problem TLC shares with most other analytical techniques is the need to calibrate with standards of the same compounds as those to be determined. This requirement is alleviated somewhat by the latroscan technique. This is an instrumental system which substitutes silica-coated rods for TLC plates. The rods are passed through a flame ionization detector to visualize the zones after development. Since the FID response is proportional to carbon content rather than chemical functionality, roughly quantitative work may be performed without external calibration (5). 

For the quantitation of chromatograms, the development condition found by the conventional TLC was transferred to another TLC system which uses a thin quartz rod coated with silica gel of 75 p thickness and may be called thin-layer-FID chromatography . The TLC system is equipped with a flame ionization detector (FID) and commercially available as a complete set (Iatron thinchrograph model TFG-10, Iatron Co., Ltd., Tokyo). The principle of sample scanning and device for FID are almost the same as those worked out by Padley57), Szakasits et al, and... 

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