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Expression of Cloned DNA

The choice of a particular expression. system depends on a number of factors. Protein yield requirements for biological activity, and compatibility of the expressed protein with the [Pg.168]


Yeast. Advances in studies of eukaryotic gene expression systems have been made with lower eukaryotes such as yeast. Protoplasts of yeast may be transformed by DNA carried on a bacterial plasmid at a frequency, indicated by the use of markers on the foreign DNA, of 1 per 10 viable cells. Alternatively a yeast plasmid vector may be used. However, so far, expression of cloned DNA in yeast appears to be non-specific. ... [Pg.145]

Su, X., Prestwood, A.K. and McGraw, R.A. (1991) Cloning and expression of complementary DNA encoding an antigen of Trichinella spiralis. Molecular and Biochemical Parasitology 45, 331—336. [Pg.128]

Cloning and expression of the DNA coding for luciferases from different luminescent organisms have provided the basis for the rapid expansion in the knowledge of molecular biology of luminescence [195,196] and in its use. It was found... [Pg.263]

Kubo, T., Fukuda, K., Mikami, A., Maeda, A., Takahashi, H., Mishina, M., Haga, T., Haga, K., Ichiyama, A., Kangawa, K. Cloning, sequencing, and expression of complementary DNA encoding the muscarinic acetylcholine receptor, Nature 1986, 323, 411-416. [Pg.452]

Panicker MM, Parker I, Miledi R. Receptors of the serotonin 1C subtype expressed from cloned DNA mediate the closing of K+ membrane channels encoded by brain mRNA. Proc Natl Acad Sci USA 1991 88 2560-2562. [Pg.197]

DNA (section 2.4.1) than eukaryotic RNA (section 2.4.2). The result will be the construction of a collection of cloned DNA fragments propagated in bacteria that is called the genomic library. This library should contain representatives of every sequence in the chromosome of a prokaryotic cell and every expressed gene in the case of a eukaryotic cell. The final step consists of the screening of every recombinant clone to identify the required gene(s). [Pg.421]

Romkes, M., Faletto, M. B., Blaisdell, J. A., Raucy, J. L., and Goldstein, J. A. (1991) Cloning and expression of complementary DNAs for multiple members of the human cytochrome P450IIC subfamily. Biochemistry 30, 3247-3255. [Pg.32]

Maipartida F, Hopwood DA. Molecular cloning of the whole biosynthetic pathway of a Strepiomyces antibiotic and its expression in heterologous host. Nature 1984 309 462-464-Chen CW, Lin H-F, jCuo CL, T ai H-L, Tsai JF-Y, Cloning and expression of a DNA sequence conferring cephamycin C production. Bio/TechiKtl 1988 6 1222-1224. [Pg.750]


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