Cloning phage

Y X cloned phage cDNA library I I against biotinylated small... 

For extensive reviews, particularly for the beginnings of gene cloning, phage display, applications in biotechnology, and recombinant antibody development, see [72, 155-157]. 

Fig. 9. Mutagenesis by a synthetic oligonucleotide of a cloned sequence available in single-stranded form (a) single-stranded M13 template containing uracil (U) residues (b) double-stranded product, uracil residues are not mutagenic (c) strong selection for M13 phages containing mutation of interest (23).

Fig. 5. Generation of mutants using single-stranded DNA. After cloning the target gene into M13, the phage is propagated in the E. coll dut, ung strain of E.

Smith, G.P. Filamentous fusion phage novel expression vectors that display cloned antigens on the virion surface. Science 228 1315-1317, 1985. 

Wurtzel, E.T. et al.. Two-step cloning of the Escherichia coh regnlatory gene ompB, employing phage Mn, J. Mol. Appl. Genet. 1, 61, 1981. 

Yanisch-Perron, C., Vieira, J. and Messing, J. 1985. Improved M13 phage cloning vectors and host strains nucleotide sequences of the M13mpl8 and pUCl9 vectors. Gene 33 103-1 19. 

Figure 5.8. Schematic illustration of T7 bacteriophage display system. The gene encoding the protein of interest is fused to the gene 10 protein that encodes a coat protein in the head of the phage. Cloning is to the C-terminus of coat protein 10. Figure adapted from Sche et al. (1999).

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