Complementary DNA cloning

Rebmann G. Hertig C. Bull J. Mauch F. Dudler R. (1991) Complementary DNA cloning and sequence analysis of a pathogen-induced putative peroxidase from rice / / Plant Mol. Biol. V. 16. P. 329-331. 

The G-protein that has been termed Gp, and that is linked to phospholipase C activation, may in fact be Gaj 2 or Gc. 3. Ga is designated as the G-protein responsible for activation of phospholipase A2, which results in arachidonic acid release. Some experimental evidence indicates that, at least in HL-60 cells, different agonists can preferentially activate different phospholipases, and some of these are responsible for the activation of secretion. In neutrophils, the two pertussis-toxin-sensitive Ga-proteins (Gaj-2 and G j 3) have been identified by peptide mapping of proteolytic digests of the proteins, by peptide sequencing and by immunoblotting. Complementary-DNA clones for the mRNA of these two molecules have also been isolated from an HL-60 cDNA library. Gai-2 is five to ten times more abundant than Gai.3, the former component comprising 3% of the total plasma membrane proteins. It is possible that these two different Ga-subunits are coupled to different phospholipases (e.g. phospholipases C and D). Pertussis toxin inhibits the secretion of O2 after stimulation of neutrophils by fMet-Leu-Phe, but pertussis-toxin-insensitive G-proteins are also present in neutrophils. These may be members of the Gq family and may be involved in the activation of phospholipase Cp (see 6.3.1). 

Mehdy, M.C. Lamb, C.J. (1987). Chalcone isomerase complementary DNA cloning and messenger RNA induction by fungal elicitor, wounding and infection. The EMBO Journal 6, 1527-34. 

Payne, G., Ahl, P., Moyer, M., Harper, A., Beck, L., Meins, F. Ryals, J. (1990a). Isolation of complementary DNA clones encoding pathogenesis-related proteins P and Q, two acidic chitinases from tobacco. Proceedings of the National Academy of Sciences (USA) 87, 98-102. 

Kdly MD, Essex DW, Shapiro SS, Mdoni FJ, Drudc T, Huebner K, Konkle BA. Complementary DNA cloning of the altemativdy expressed endothdial cell glycoprotdn Ibb (GPlbb) and localization of the GPlbb gene to chromosome 22. J.Clin-lnvesL 1994 93 2417-2424. 

J.P. Hardwick, H.V. Gelboin, and F.J. Gonzalez (1989). Human NADPH-P450 oxidoreductase Complementary DNA cloning, sequence and... 

Kwok SC, et al. Nucleotide sequence of a full-length complementary DNA clone and amino add sequence of human phenylalanine hydroxylase. Biochemistry. 1985 24(3) 556-61. 

G. Robbelen, R.K. Downey and A. Ashri, McGraw-Hill, New York, pp. 260-292. Knutzon, D.S., Sherer, D.E. and Schreckengost, W.E. (1991) Nucleotide sequence of a complementary DNA clone encoding stearoyl-acyl-carrier protein desaturase from castor bean, Ricinus communis. Plant Physiol. 96, 344-345. 

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