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Plasmids Are Used to Clone Small Pieces of DNA

In a simple procedure for DNA cloning, an autonomously replicating plasmid and insert DNA are cut with a restriction enzyme and then the pieces are annealed and covalently joined by the action of DNA ligase. The resulting recombinant molecules are then transfected into E. coli, where they replicate. When plasmid vectors are used, a population of permeabilized cells is bathed in the plasmid DNA containing the inserted DNA. Because only a small number of cells become transfected by this procedure, a way to se- [Pg.683]

Normal medium plus penicillin plus tetracycline [Pg.684]

Bacteriophage A Vectors Are Useful for Cloning Larger DNA Segments [Pg.685]

Bacteriophage A vectors that accommodate foreign DNA fragments generated by a variety of restriction endonucleases have been constructed. The recombinant DNA molecules that incorporate some of these vectors can be introduced directly into E. coli by transfection. Alternatively, recombinant DNA molecules can be packaged into phage particles and subsequently infected into suitable host cells. [Pg.686]

Shuttle Vectors Can Be Cloned into Cells of Different Species [Pg.686]


See other pages where Plasmids Are Used to Clone Small Pieces of DNA is mentioned: [Pg.678]    [Pg.683]   


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Clones. DNA

Cloning of DNA

Cloning plasmids

DNA cloning

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Of plasmids

Pieces

Plasmid DNA

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