Chromosome maps

Lederberg s construction of a branched chromosome map on the basis of linkage analysis was mentioned in the last section. As late as the 1950s, Delbruck continued to hope that the physical order of loci on chromosomes would contradict their order as determined by linkage analysis this was supposed to show the limitation of reductionist explanation in biology.12... 

Shibui, A., et al. Isolation and chromosomal mapping of a novel human gene showing homology to Na+/P04 cotransporter./. Hum. 

Meyne, J. (1993) Chromosome mapping by fluorescent in situ hybridization. In Methods in Nonradioactive Detection (G.C. Howard, ed.), pp. 263-268. Appleton Lange, Norwalk, Connecticut. 

Yasuhiko M, Appels R. Direct chromosome mapping of plant genes by in situ polymerase chain reaction (in situ PCR). Chromosome Res 1996 4 401 404. 

Cahilly-Snyder, L., et ah. Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line. Somat Cell Mol Genet, 1987, 13(3), 235-44. 

At the upper part of the search page, the chromosomal viewer, the phylogenetic map button, and the content search area are shown. As an initial value, all GPCR genes of Level A are displayed at chromosomal map. 

Yokota H, Fernandez-Salguero P, Furuya H et al. cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria. J Biol Chem 1994 269 23192-23196. 

Haefliger JA, Bruzzone R, Jenkins NA, Gilbert DJ, Copeland NG, Paul DL Four novel members of the connexin family of gap junction proteins Molecular cloning, expresion and chromosome mapping. J Biol Chem 1990 116 187-194. 

Thomas H. Morgan Physiology/Medicine Chromosome theory and chromosome maps... 

Figure 7-29 Assembly sequence for bacteriophage T4 with details for the tail. The numbers refer to the genes in the T4 chromosome map (Fig. 26-2). A "P" after the number indicates that the protein gene product is incorporated into the phage tail. Other numbers indicate gene products that are thought to have essential catalytic functions in the assembly process. Adapted from King and Mykolajewycz236 and Kikuchi and King.214...

Figure 25-1 Aromatic biosynthesis by the shikimate pathway. The symbols for several of the genes coding for the required enzymes are indicated. Their locations on the E. coli chromosome map are shown in Fig. 26-4. The aminoshikimate pathway which is initiated through 4-aminoDAHP leads to rifamycin and many other nitrogen-containing products.

The product of step o is known as isochoris-mate.55 56 Isochorismate gives rise to a variety of products including vitamin K, salicylic acid,56a the iron chelator enterobactin (Fig. 16-1), and other siderophores. These are formed in E. coli via 2,3-dihy-droxybenzoate as indicated in Fig. 25-2.57-59a The genes (ent) for the requisite enzymes are clustered at 14 min on the E. coli chromosome map (Fig. 26-4). 

DNA has been substituted in one strand.98 Since distances can be measured accurately on the electron micrographs, rather precise ( 50-100 bp) physical maps can be obtained. The chromosome map of phage K was mapped in this way initially now its complete nucleotide sequence is known. Another electron microscopic method is useful for location of AT-rich regions that denature readily. In a suitable concentration of formamide these regions melt to form visible single-stranded denaturation loops similar to the bubbles in Figure 26-6. 

I) [ May 199(h "Five-year Plan Goes to Capitol Hill. Projects begun to mark gene sites on chromosome maps as sites of mRNA expression. 

I99 Human chromosome mapping data repository, GDB. esiablished. See... 

K21. Kozyraki, R., Kristiansen, M., Silahtaroglu, A., Hansen, C., Jacobsen, C., Tommerup, N., Verroust, P. J., and Moestrup, S. K., The human intrinsic factor-vitamin B12 receptor, cubilin Molecular characterization and chromosomal mapping of the gene to lOp within the autosomal recessive megaloblastic anemia (MGA1) region. Blood 91, 3593-3600 (1998). 

Sher T, YiH-F, McBride OW, et al. 1993. cDNAcloning, chromosomal mapping, and fimctbnal characterization of the human peroxisome proliferator activated receptor. Biochemistry 325598-5604. 

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