Chromatography multiple detection

Aranda, M., Morlock, G. (2006) Simultaneous determination of riboflavin, pyridoxine, nicotinamide, caffeine and taurine in energy drinks by planar chromatography-multiple detection with confirmation by electrospray ionization mass spectrometry.Journal of Chromatography. A, 1131,253-260. 

The method for chloroacetanilide soil metabolites in water determines concentrations of ethanesulfonic acid (ESA) and oxanilic acid (OXA) metabolites of alachlor, acetochlor, and metolachlor in surface water and groundwater samples by direct aqueous injection LC/MS/MS. After injection, compounds are separated by reversed-phase HPLC and introduced into the mass spectrometer with a TurboIonSpray atmospheric pressure ionization (API) interface. Using direct aqueous injection without prior SPE and/or concentration minimizes losses and greatly simplifies the analytical procedure. Standard addition experiments can be used to check for matrix effects. With multiple-reaction monitoring in the negative electrospray ionization mode, LC/MS/MS provides superior specificity and sensitivity compared with conventional liquid chromatography/mass spectrometry (LC/MS) or liquid chromatography/ultraviolet detection (LC/UV), and the need for a confirmatory method is eliminated. In summary,... 

Pasch, H. (2004). Characterization of Polymer Heterogeneity by 2DLC. In Striegel, A.M., editor.Multiple Detection in Size-Exclusion Chromatography. ACS Symposium Series 893, American Chemical Society Washington, D.C. 

Sadi, . . M., Vonderheide, A. P., Becker, J. S. and Caruso, J., Multiple Detection Size-Exclusion Chromatography, ACS Symposium Series 893, American Chemical Society, Washington, DC, 168 (2004). Szpunar, J. and Lobinski, R., Hyphenated Techniques in Speciation Analysis. R. M. Smith (ed.) RSC Chromatic Monographs, Cambridge (2003). 

Ikarashi et al. reported the development of a liquid chromatography multiple electrochemical detector (LCMC) and its application in neuroscience [197]. The system developed consisted of four parallel liquid chromatographic systems equipped with multiple electrochemical detectors, and the fourth was used for the assay of acetylcholine with a detection limit of 0.1-0.4 pmol. 

Identification and quantitation can be done with model compounds or with specific detection, like Fourier transform infrared or other kinds of multiple detection. However, 2D chromatography is also extremely useful for fingerprinting and visual comparison. 

Detection and quantitation of PAH-derived DNA adducts requires authentic synthetic standards to act as internal standards and therefore a knowledge of adduct structure. Adduct structure can often be used to provide information on the mechanism of PAH activation that must have occurred. Unfortunately, this is quite challenging since PAH-DNA adducts can be difficult to detect and quantitate since they may be present in only 1 108 nucleotides. State-of-the art methods requiring stable isotope dilution liquid chromatography/multiple reaction monitoring/mass spectrometry (LC/MRM/MS) are necessary to provide sufficient sensitivity to detect these adducts reliably. Additionally, PAH-DNA adducts have different rates of repair and yield different rates of mutation. Thus, each adduct must be assessed so that it can be ranked according to its half-life and miscoding potential. 

Using analytical pyrolysis for such samples would be just one component of a systems approach to multimedia analysis, a multifaceted approach to the examination of environmental samples centered upon the use of a purge-and-trap/head-space/pyrolysis/gas chromatography system utilizing multiple detection systems. Although the development of such an analytical scheme was under discussion more than 10 years ago, no analytical method using Py-MS, Py-GC, or Py-GC/MS has yet been promulgated. 

Christiansen AL, Rasmussen KE (1983) Screening of hallucinogenic mushrooms with high performance liquid chromatography and multiple detection. J Chromatogr 270 293-299... 

Striegel, A.M. Multiple detection in size-exclusion chromatography of macromolecules. Anal. Chem. 2005, 77, 104A-114A. 

Recent development in liquid chromatography analysis of complex polymers shows a clear trend to combine more than one LC separation mechanism/tech-nique together with multiple detection techniques. It is a quite natural direction for the analysis of complex polymers with multivariate distributions in molecular characteristics. The coupled LC techniques have gained wide attention recently in the characterization of complex polymers and there are a number of monographs and reviews on this topic [4,8-13]. In this chapter, recent advances in LC separation of polymers are reviewed. References are generally restricted to the works published after 1995 since most of the works prior to the mid-1990s have been well summarized cdready [8,10,11,13]. 

Quantitative Determination of Styrene-7, 8-oxide in Blood by Combined Gas Chromatography-Multiple Ion Detection Mass Fragmentography J. Chromatogr. 196(2) 314-318 (1980) ... 

Analysis of Multiple Detection Data in Analysis of Polycarbonates, Hewlett Packard HPLC Application 87-4, Gel Permeation Chromatography, 1987. 

Pasch H (2004) Characterization of polymer heterogeneity by 2D-LC. In Striegel AM (ed) multiple detection in size-exclusion chromatography. ACS Symposium Series, vol 893. American Chemical Society, Washington, DC... 

Reed WR Fundamentals of static light scattering and viscom-etry in SEC and related methods. In Striegel AM, editor. Multiple Detection Size-Exclusion Chromatography. ACS Symposium Series 893. Washington, DC American Chemical Society 2004. 

I.S. Krull, M. Swartz, and J.N. Driscoll. Multiple detection in gas chromatography. In Advances in Chromatography, Volume 24, Ed. by J.C. Giddings, E. Grushka, J. Cazes, and PR. Brown, Marcel Dekker, New York, 1984, Chapter 8. 

Mateus-Avois, L., Mangin, R, and Saugy, M. (2005) Use of ion trap gas chromatography-multiple mass spectrometry for the detection and confirmation of 3 hydroxystanozolol at trace levels in urine for doping control. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 816,193-201. 

Adapted irom Striegel, A. M. In Multiple Detection in Size Exclusion Chromatography Siueg(t, A. M., Ed. ACS Symposium Series 893 American Chemical Society Washington, DC, 2005, with permission. Copyright 2005 American Chemical Society. 

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