Chromatography Forum

Parts of this work were presented at the 1996 American Chemical Society Mid Atlantic Regional Meeting, Student Award Symposium, The Chromatography Forum of Delaware Valley, May 24,1996, Villanova University, Villanova, PA, Abstract no. 363. 

This text represents a blending of the basic theories of chromatography with the experiences of each author. It is the product of a Gas Chromatography Short Course which has been presented annually in Philadelphia by the Chromatography Forum of Delaware Valley. The course has been offered each year with the highest level of participation. It has been structured for not only beginners but for existing workers in the field and specialists of other fields who wish and need to know more about this powerful... 

Any book, whether a textbook or reference work can not be produced without the help and cooperation of many people. I wish to acknowledge the members of the Chromatography Forum of the Delaware Valley, the contributing authors for their cooperation, professional attitude, and unselfish availability of their time my son Duane for the drawings in several of the chapters and the creation of the cover drawing, Dr. Gerald R. Umbreit for assistance and cooperation beyond those of a contributor, and Drs. Mary A. Kaiser, Matthew J. O Brien, and John F. Wojcik for helping... 

A discussion of the history of liquid chromatography is beyond the scope of this volume an excellent treatment has been published by Ettre (3). However, some perspective on the beginnings of modern liquid chromatography is useful for an understanding of the technique as now practiced. The first comprehensive publication on the technique was a book edited by Kirkland (4), which grew from a short course offered by the Chromatography Forum of the Delaware Valley. This then laid the foundation for the definitive book, now in its second edition, Introduction to Modern Liquid Chromatography (2). 

Jacobs, W. A., and Keddington, J. (1983). Minnesott Chromatography Forum, Minneapolis, MN, Poster 19,... 

Chromatography Forum http //www.chromforum.org Google Group sci.chem.analytical ... 

P. Wang and B. Glassbrenner (1988). Paper presented at Minnesota Chromatography Forum 27, Bloomington, MN. 

Johnson, Y. An FDA Perspective on Method Transfer of Chromatographic Analytical Methods in the Pharmaceutical Industry. The Chromatography Forum of Delaware, October Meeting, 2004. 

R-13 Tsuge S. Characterization of polymers by high-resolution pyrolysis-gas chromatography with fiised-silica capillary columns. Chromatography Forum 1986 1 44-51. 

PC. Uden. Element-selective chromatographic detection by atomic emission spectroscopy. Chromatography Forum, 17 (Nov/Dec, 1986). 

Gressin, J. C., Online process chromatography a useful tool in biochemical processes, Biotech. Forum, 5, 38, 1988. 

B. Jones, K. Markides, et al., Contemporary capillary column tech- j nology for chromatography, Chromatogr. Forum, 38-44 (May-June 1986). 

G. Guiochon and H. Colin, Theoretical concepts and optimization in preparative scale liquid chromatography, Chromatogr. Forum., 21, (Sept.-Oct. 1986). 

D. Helton, M. Ready, and D. Sacks, Advantages of a common column and common mobile phase system for steroid analysis by normal phase high-performance liquid chromatography by W. F. Beyer—comments received, Pharmacopeia/Forum, 2794 (1983). 

Proc. First International Particle Technology Forum, vol. 2, AIChE, Denver, 1994, p. 178), whereas surface energy can be characterized by inverse gas chromatography and other adsorption techniques. Particle yield pressures and elastic moduli of the powder feeds can also be determined by uniaxial compaction experiments which monitor deformation and pressure throughout the compaction cycles. In addition, rate effects are investigated, as plastic and elastic properties can be rate-dependent for some materials. 

Once again, the wide depth and breadth of knowledge regarding HPLC method development and assay optimization available in the literature cannot be covered here. The first suggestion is to check the literature for well-documented assays of known or related compounds. In many cases, the method used for HPLC/UV provides a helpful starting point for LC/MS methods with the same compound. In the interest of brevity, method development discussions are limited to reverse-phase separations. The unique requirements of normal phase and chiral chromatography are best discussed in other forums. 

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