Chromatography elution profiles

Figure 6A-C. High-performance anion-exchange chromatography elution profile of isolated modified hairy regions without addition of enzymes (A), with Biopectinase OS (B) and with rhamnogalacturonase-containing culture filtrate from an A. awamori multicopy transformant (C). /xC ftCoulomb- Data taken from Ref. 6.

Fig. 2. (a)-(c) Q Sepharose chromatography elution profiles during RNAP purification. Chromatography runs are described in the text, (d) Coomassie stained SDS-PAGE analysis of RNAPs from Sulfolobus acidocaldarius (S.a.), Sulfolobus shibatae (S.s.), and Pyrococcus furiosus (P.f.). 

FIGURE 10.5 Elution profile on OH-B12 treated by microwave heating for 6 min during silica gel 60 column chromatography. Fifty milliliters of the treated OH-B12 solution (5 mmol/1) was evaporated to dryness and dissolved in a small amount of w-butanol/2-pro-panol/water (10 7 10, v/v) as a solvent. The concentrated solution was put on a column (1.4 X 15.0 cm) of silica gel 60 equilibrated with the same solvent and eluted with the same solvent in the dark. The eluate was collected at 4.0 ml with a fraction collector. Fractions I to V were pooled, evaporated to dryness, dissolved with a small amount of distilled water, and analyzed with silica gel TLC. Inset represents the mobile pattern of the OH-B12 degradation products of fractions I to V on the TLC plate. Data are typical, taken from one of five experiments. (Reprinted with permission from Watanabe, F. et al., J. Agric. Food Chem., 46, 5177-5180, 1998. Copyright (1998) American Chemical Society.)... 

P.J. Gemperline, A priori estimates of the elution profiles of the pure components in overlapped liquid chromatography peaks using target factor analysis. J. Chem. Inf. Comput. Sci., 24 (1984) 206-212. 

Analytical size exclusion chromatography was performed on an Akta Purifier (Pharmacia) using a 24-ml Superose 6 HR 10/30 column (Pharmacia). 250-pl samples in AP-buffer (20mM Tris buffer, pH 8.0, 100 mM NaCl), with or without 0.1 % P-DM, were injected onto the column equilibrated with the same buffer and eluted at a flow rate of 0.3 ml min 1. Elution profiles were recorded at 220, 280 and 674 nm. 

A typical elution profile the separation of saturated esters by gas-liquid chromatography 1. methyl formate 2. methyl acetate 3. ethyl formate 4. ethyl acetate 5. -propyl formate 6. iso-propyl acetate 7. w-butyl formate 8. sec-butyl acetate 9. iso-butyl acetate 10. n-butyl acetate... 

VDU screen via suitable electronic amplifying circuitry where the data are presented in the form of an elution profile. Although there are a dozen or more types of detector available for gas chromatography, only those based on thermal conductivity, flame ionization, electron-capture and perhaps flame emission and electrolytic conductivity are widely used. The interfacing of gas chromatographs with infrared and mass spectrometers, so-called hyphenated techniques, is described on p. 114 etseq. Some detector characteristics are summarized in Table 4.11. 

In common with M. macedonicus, urine from male M. spretus also demonstrated a MUP-sized band following gel electrophoresis. The proteins within this band were analysed by high resolution anion exchange chromatography and electrospray ionisation mass spectrometry (ESI-MS). The former technique produced an elution profile consisting of just three peaks, in contrast to both the more complex patterns observed previously from M. m. domesticus and the single major peak in M. macedonicus. Furthermore, similar analyses from five individual males resulted in near... 

According to gel permeation chromatography (GPC) data, the molecular weight of SPP increased after X-ray exposures both in air and in vacuum. The elution profiles in both cases were very similar, indicating that the coupling of APSQ and DNQ or the condensation of APSQ occurs irrespective of the ambient. 

Size exclusion chromatography (SEC) polymer elution profiles yield information regarding the molecular size distributions of polydisperse macromolecules. Polymer molecular weight distribution (MWD) represents an intrinsic property which provides direct correlation with many end-use physical properties and a universal criterion for polymer characterization (1). In order to convert elution profiles or chromatograms into MWD information proper calibration methods are required. SEC molecular weight calibration techniques represent experimental approaches for transformation of polymer elution profiles into MWD information and are dependent upon instrumentation, columns, and the polymer/solvent system under study. 

Figure 2 (right). Reverse-phase HPLC elution profile of the tumor-localizing fraction of HPD isolated by non-aqueous gel exclusion chromatography. (B) Hydrolysis of this material in 50% aqueous THF containing IM HCl, at 37 °C for 24 hours. (C) After hydrolysis in IM NaOH, 50% aqueous THF under the same condition. The major porphyrins resulted are hematoporphyrin (HP), hydroxyvinyl deutero-porphyrin (HVD), and protoporphyrin (PP). 

Estimation of Polymer Sizes by Gel Permeation Chromatography. The copolymer (1 mg) was dissolved in 1 ml of phosphate buffered saline (PBS), pH 7.4, and applied to a column of Sephacryl S-300 (1 X 108 cm) or Sephacryl S-400 (1 x 114 cm). The column was eluted with PBS at a flow rate of 0.2 ml/min. The elution profile of the copolmer was monitored by its absorbance at 214 nm. Bovine serum albumin (BSA) was chromatographed for comparative purposes and polyacrylamide standards (Modchrom, Inc.) were used. 

Figure 4. Elution profile of the 2 - and 5 -0-glucosyltransferases after chromatography on Brown 10X agarose column using pH-salt gradient. Insert autoradiographed enzyme reaction products.

Reversed-phase chromatography is often used to separate both neutral and ionic organic compounds. In this section, some important aspects for the understanding of the behavior of ionic compounds in reversed-phase chromatography are discussed. The important concepts introduced here are the electrical double layer and the electrostatic surface potential. It will be shown that they are essential for the understanding of the elution profile of ionic compounds. These concepts are further explored in the next section where theoretical models for ion-pair chromatography are discussed. 

Fig. 6. Elution profiles of three alkyl 2-acetamido-2-deoxy- -D-glucopyranosides 4a (a. n=8 b. n=ll c. n=14) in gel filtration chromatography on sepharose 4B (exclusion 2.10 Da, exclusion volume 10 mL) monitored by and radioactivity countings (normed to 1.0 for reason of convenience). ( ) H countings of H-labelled phosphatidyl choline (x) C...

Examples for and have been observed under certain experimental conditions for reactive and/or strained chiral oxiranes which were separated by complexation gas chromatography (Figure 21)133. The first eluted peak was diminished in the separation of racemic 2-methyl-3-phenylo.xirane. In this case two enantioselective processes are mediated by the chiral metal chelate, i.e., chromatographic resolution and kinetic resolution (in favor of the first eluted enantiomer). Since two enantioselective processes are involved, the elution profile will be the same svhen the chirality of the metal chelate is inverted. 

The data in the upper panel of Figure 9 show the elution profile obtained from the separation of acid extracts of uncooked, cooked, and cooked/stored meat by size exclusion chromatography. Uncooked meat has the greatest... 

Recovery, Purity and Amino Acid Composition of CMP. The elution profiles of the CMP powders obtained on size exclusion chromatography 23) are shown in Figure 6. Both CMP isolated from WPC and whey did not contain major... 

Figure 5. Composite MWD diagram showing the calculated distributions of four of the five master fractions obtained from preparative chromatography of organosolv aspen lignin (fraction number 4 to 1 from left to right). The Mw values for the master fractions from left to right are 1,110, 1,310, 2,420, and 8,050, respectively. The insert shows the elution profile of organosolv aspen lignin from the YMC preparative /z-Styragel column (5 x 200 cm). The 30 fractions collected were pooled into the five master fractions shown.

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