High-pressure liquid chromatography elution profile

Figure 4.22. Separation of PTH-Amino Acids. PTH-amino acids can be rapidly separated by high-pressure liquid chromatography (HPLC). In this HPLC profile, a mixture of PTH-amino acids is clearly resolved into its components. An unknovm amino acid can be identified by its elution position relative to the known ones.

Figure 21.21. Gradient devices used in liquid chromatography. A Simple mixing device for gradient elution, gradient formed on low-pressure side of pump. Ri is the flow rate of A into the flask. Ra is the flow rate of A + B to column. B Gradient profiles obtained from A. C Schematic for two-pump gradient chromatograph. Gradient formed on high-pressure side of pump.

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