Chromatography profile

Fig. 2.54. High-performance liquid chromatography profile of a peppermint sample (gradient no.l), extracted with ethyl ether (a) and ethanol (b). Chromatographic profile of a sample extracted with ethanol (gradient no.2) (c). Detection at 320 nm. For peak identification see Table 2.55. Reprinted...

Figure 7 Size exclusion chromatography profiles of coupled polystyrene before and after y-irradia-tioninMTHFat295 K.

Studies on HPLC with iridoids of Apoc)maceae focus mainly on the separation of components from extracts or fraction. The chromatography profile, the identification and quantification of these terpenes in the extracts are described. Table 4 shows the principal references on iridoids isolated from Apoc)maceae by HPLC, MPLC and LPLC. 

Figure 4 Get permeation chromatography profiles of hyaturonan <0.3 mgfmL) after 0 ( ), 20 ( ), 40 (A), and 60 (P minutes of treatment with hyalmnidase (0.33 IU/mL). (from Ref. 139.)...

The thin-layer chromatography profile alone will provide a quite accurate evaluation of the extent of the cleavage reaction. If a more quantitative assay is desired, then another plate—usually a preparative type—can be run and individual components can be identified by TNS spray, removed by scraping, and extracted with chloroform-methanol-water (1 2 0.8, v/v) and analyzed after phasing the desired compound into chloroform. Then, the yield of the phosphorus-containing compound can be determined. The methyl esters can be assayed by GC-MS. The presumed alkylglycerophosphocholine then can be studied further. 

Figure 9.10 Progressive formation of the isotachic train in displacement chromatography. Profiles along the column. Solute A1 (dash-dot), solute A2 (dash-dot-dot), solute A3 (solid), solute A4 (dash). H.-K. Kkee and N.R. Amundson, AICHE J, 28 (1982) 423 (Fig. 10). Reproduced hy permission of the American Institute of Chemical Engineers. 1982 AIChE. All rights reserved.

Figure 2. High performance liquid chromatography profile of centrifuged whole broth and mycelium extract. Column Waters Assoc. 1-125 protein analysis column (30 cm x 7.8 mm). Mobile phase 0.05 M phosphate buffer, pH 6.9. Flow rate 1.0 ml/min, Detector UV at 210 nm.

Figure 6. High performance liquid chromatography profile of Durapore processing (condition similar to, as in Figure 1).

Figure 2 Size-exclusion chromatography profile of IVEAX after the action of the SR3 cellular extract...

A. Stewart, H.M. Kalisz, A. Isacchi, Inhibitor affinity chromatography profiling the specific reactivity of the proteome with immobilized molecules, Proteomics 2003, 3(7), 1287-1298. 

Lee M.L., Smith D.L. and Freeman L.R. (1979) High resolution gas chromatography profiles of volatile organic compounds produced by micro-organisms at refrigerated temperatures. Appl. Environ. Microbiol., 57, 85-90. 

Martin, R, A. and S. P. Lynch, Cardenolide content and thin-layer chromatography profiles of monarch butterflies, Danaus plexippus L., and their larval host-plant milkweed, Asclepias asperula subsp. capricornu (Woods.) Woods., in North Central Texas, J. Chem. Ecol., 14, 295-318 (1988). 

Brower, L.P., J.N. Seiber, C.J. Nelson, S.P. Lynch, and N.N. Holland Plant Determined Variation in the Cardenolide Content, Thin-Layer Chromatography Profiles and Emetic Potency of Monarch Butterflies, Danaus plexippus L. Reared on Milkweed Plants in California. 2. Asclepias speciosa. J. Chem. Ecol. 10, 601-639 (1984). 

Fig. 3.3. Partial silver-ion high-performance liquid chromatography profile of (A) the methyl esters of a commercial CLA isomer mixture, (B) the l2-iso-merized product of (A), and (C) the l2-isomerized product of (A) co-injected with cheese total lipid fatty acid methyl esters. Ultraviolet detection at 233 nm. (Published with permission from Ref. 14 and redrawn from original.)...

Brower, L. P., Seiber, J. N., Nelson, C. J., Lynch, S. P. andTuskes, P. M. (1982)Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies, Danaus plexippus, reared on the milkweed, Asclepias eriocarpa, in California. J. Chem. Ecol., 8, 579-633. 

Figure 4. Analysis of the different polyphosphoinositides extracted from P-labelled cells. (A) Schematic representation of the expected separation of a mixture of P-labelled phospholipids by thin layer chromatography (TLC). Plates are silica gel 60 and the solvent for phosphoinositide separation is a mixture of CHCI3, CH3COCH3, CH3OH, CH3COOH and H O (80 30 26 24 14, v/v). MP, major phospholipids (phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine). (B) Typical high-performance liquid chromatography profile showing the separation of the various phosphoinositides from a mixture of P-labelled phosphoinositides. A specific gradient must be used to separate PtdIns(4)P and PtdIns(5)P (Rameh et ai, 1997 Niebhur et al., 2002).

Purification of the nonfusion rCRALBP produced with the pET-3a vector is accomplished essentially as described by Saari and Bredberg (25) for bovine-retinal CRALBP. (Ref. 25 includes representative chromatography profiles.) All rCRALBP purification steps must be carried out under dim red illumination to prevent photodecomposition of the retinoids. 

Maga, J.A. and K. Lxrrenz, The effect of flavor enhancers on direct headspace gas-liquid chromatography profiles of beef broth, J. Food Sci., yi, p. 963, 1972. 

Graham, T.L., A rapid high-resolution high performance hquid chromatography profiling procedure for plant and microbial aromatic secondary metabohtes. Plant Physiol., 95, 584-593, 1991a. 

Figure 2. Gel permeation chromatography profile of starch hydrolysate using multi-enzyme regime of Birmingham 2 method. 

Figure 2 Mono Q column chromatography profile Figure 3...

Demin P, Reynaud D, Pace-Asciak CR. Extractive derivatization of the 12-lipoxygenase products, hepoxilins, and related compounds into fluorescent anthryl esters for their complete high-performance liquid chromatography profiling in biological systems. Anal Biochem 1995 226 252-255. 

Viarma Braga, M.C., Konno, K., Portaro, F.C.V., de Freitas, J.C., Yamane, T., Olivera, B.M., and Pimenta, D.C. (2005) Mass spectrometric and high performance liquid chromatography profiling of the venom of the Brazilian vermivorous mollusk Conus regius feeding behaviour and identification of one novel conotoxin. Toxicon, 45,113-122. 

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