Chromatographic Separation and Identification

A powerful new method of additive extraction has been developed termed supercritical fluid extraction/FTlR 

---

Benschop, H.P., Konings, C.A.G., De Jong, L.PA. (1981). Gas chromatographic separation and identification of the four stereoisomers of 1,2,2-trimethylpropyl methylphosphono-fluoridate (soman). Stereospecificity of in vitro detoxification reactions. J. Am. Chem. Soc. 103 4260. 

The reagent forms colored esters with alcohols which are useful for chromatographic separation and identification. Compare 4 -NitroazobenMn -4-CHrboxylic acid chloride. 

Finally, it should be mentioned that several analytical methods for chromatographic separation and identification of pyridazines have been developed.Also polarographic determination and other analytical methods were applied. Pyridazines have been proposed as analytical reagents for the spectrophotometric determination of iron or for the qualitative determination of sodium. ... 

Peptide mapping, after selective enzymatically or chemically induced fragmentation of the product into peptides, followed by chromatographic separation and identification by sequencing techniques, UV-absorption, mass spectrometry or other alternatives, serves as a combination technique by which various types of biopharmaceuticals may be thoroughly characterized. An indication of genetic stabihty can be demonstrated by comparison with reference materials, as incorrect amino acids resulting from mutation or mistranslation of the cDNA sequence can be determined. 

Abdel-Kader, M. H. K., Stiles, D. A., and Ragab, M. T. H., Thin-layer chromatographic separation and identification of carbofuran and two metabolites and their dinitrophenyl esters, Int J. Environ. Anal. Chem., 18, 281-286, 1984. 

The low concentrations of free sterols in ocean waters require that large volumes of seawater are extracted in order to decrease the detection limit for FID detection. The structural similarity of the sterol compounds found in seawater makes demands on the chromatographic separations and identification, which may only be reasonably met with the use of the GC—MS systems (e.g. Gagosian and co-workers Saliot and co-workers). 

Sehat, N., Yurawecz, M.P., Roach, J.A.G., Mossoba, MM., Kramer, JX.G., and Ku, Y. (1998) Silver-Ion High-Perfonnance Liquid Chromatographic Separation and Identification of Conjugated Linoleic Acid Isomers. Lipids 33,217-221. 

Sehat, N., M.R Yurawecz, J.A.G. Roach, M.M. Mossoba, J.K.G. Kramer, and Y. Ku. Silver-Ion High-Performance Liquid Chromatographic Separation and Identification of Conjugated LinoleicAcid Isomers. j j 33 217-221 (1998). 

Ossipov, V. Nurmi, K. Loponen, J. Haukioja, E. Pihlaja, K. High-performance liquid chromatographic separation and identification of phenolic compounds from leaves of Betula pubescens and Betula perubda. J. Chromatogr. A, 1996, 721, 59-68. 

In addition to chromatographic separation and identification with a mass spectrometer, the IR spectra of the destruction products of polyformaldehyde were taken. For this purpose the destruction products were dissolved in carbon tetrachloride. 

Gas-Liquid Chromatographic Separation and Identification of Biologically Important IndoleaIky1amines from Human Cerebrospinal Fluid Biochem. Med. 14(2) 191-200 (1975) ... 

Gas Chromatographic Separation and Identification of Aliphatic Amines in Blood... 

Reio, L., 1970. Third supplement for paper chromatographic separation and identification of phenol derivatives and related compounds of biochemical interest using a reference-system. Journal of Chromatography. 47 60-85. 

Several reviews have been published on the analysis of phenolic acids in plants and the optimization of the extraction methods, as well as to their chromatographic separation and identification techniques. The processing methods on extraction and purification presented in this review are the most employed in phenolic acids analysis. 

Guenther ES (1943) Characteristics and uses of oQ of ohbanum. Am Perfum Ess QU Rev 45 41 3 Guthrie FE, Ringlet RL, Bowery TG (1957) Chromatographic separation and identification of some alkaloid metabolites of nicotine in certain insects. J Econ Entomol 50 821-825 Haekkinen ST, Rischer H, Laakso I, Maaheimo H, Seppaenen-Laakso T (2004) Anatalline and other methyl jasmonate-inducible nicotine alkaloids from Nicotiana tabacum cv. By-2 cell cultures. Planta Med 70 936-941... 

Acyl groups have a protective role in some synthetic reactions. Gluconic acid nitrile acetates (aldonitrile acetates) are analytically suitable sugar derivatives for gas chromatographic separation and identification of sugars. An advantage... 

Fig. VIII-6. Chromatographic separation and identification of peptides in a thermolysin digest of TNP-clupeine YI. The digestion mixture (from 2.27 p,moles of TNP-clupeine YI) was applied to a column (0.9 cm x 150 cm) of CM-cellulose. Peptides were first eluted with the starting buffer (0.1 M acetate buffer, pH 6.0 tubes 1—80), then an exponential gradient elution was applied (81—514). The mixing chamber contained 1000 ml of starting buffer and to the reservoir 0.1 M acetate buffer (pH 6.5) containing 1.0 M NaCl was added. The peptides remaining on the column were eluted with 0.1 M HCl. The flow rate of the effluent was 3.89 ml/tube/18 min. The effluent was analyzed by Sakaguchi colorimetry and spectrophotometry at 340 mp. The color yield (Sakaguchi reaction) was 101 % (tubes 1 to 555) [from Ando and Suzuki, 1967 Suzuki and Ando 1972 (2)]...

---