Chiral affinity chromatography

The ability of proteins to form enantioselective interactions with a large variety of drugs is used in chiral affinity chromatography. Protein CSPs that are most frequently used for the enantioseparation of pharmaceuticals include bovine serum albumin (BSA), human serum albumin... 

As discussed earlier, the proteins used as chiral selectors in affinity chromatography cannot be used under the high-pressure HPLC with a variety of mobile phases therefore, these proteins were immobilized with some solid support such as hydroxyethylmethacrylate, polystyrene-divinylbenzene, polyethylene fibers, and silica gel [14,15]. Avariety of techniques have been used for the immobiliza-... 

The mechanisms described above form the basis for the chromatographic modes described in Chapter 2, namely, normal-phase, reversed-phase, size-exclusion, ion-exchange, and affinity chromatographies. However, other modes that are variations of those mentioned above, such as hydrophobic-interaction chromatography (HIC), chiral, ion-exclusion, and ion-pair chromatographies are also used and will be mentioned. 

Affinity chromatography (immunoaffinity, metal affinity, chiral)... 

In Part VI, Chapter 30 is now a general introduction to separations. It includes solvent extraction and precipitation methods, an introduction to chromatography, and a new section on solid-phase extraction. Chapter 31 contains new material on molecular mass spectrometry and gas chromatography/mass spectrometry. Chapter 32 includes new sections on affinity chromatography and chiral chromatography. A section on LC/MS has been added. A new Chapter 33, Miscellaneous Separation Methods, has been included. It introduces capillary electrophoresis and field-flow fractionation. 

Hage, D. S., NoctopT. A. G.,Wainer, I. W. Characterization of the protein binding of chiral drugs by high-performance affinity chromatography. Interactions of... 

Wainer, 1. W. Enantioselective high-performance liquid affinity chromatography as a probe ofligand-biopolymer interactions an overview of a different use for high-performance liquid chromatographic chiral stationary phases,... 

The mobile phase controls the separation Whereas the stationary phase provides a media for analyte interaction, the mobile phase controls the overall separation. In HPLC method development, efforts focus on finding a set of mobile phase conditions for separating the analyte (s) from other components. Exceptions to this rule are size exclusion, chiral, and affinity chromatography. 

Imprinted materials can be used as stationary phase in affinity chromatography, especially for the separation of enantiomers. Compared to standard chiral stationary phases, these materials present the advantage of being synthesised to order for a given enantiomerically pure molecule. These chromatographic materials have allowed the separation of numerous compounds such as naprox-ene (anti-inflammatory) [139], timolol (beta-blocker) [50] and nicotine [140]. 

If you work with solutes such a steroids, isomers or naphthalene derivatives, or in a separation mode such as ion exchange, affinity chromatography, chiral chromatography etc., you should certainly check the influence of the temperature on the selectivity, but not just in the range 30 0 °C. 

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