Chicken ganglioside fractions

Figure 1A. Thin-layer chromatogram of human and chicken muscle ganglioside fractions eluted from DEAE-A 50 column...

The structure of a novel L-fucose-containing ganglioside from pig cerebellum has been established (2). 9-O-Acetyl-A-acetylneuraminic acid accounts for up to 20% of the brain ganglioside fractions from man, cow, horse, pig, sheep, cat, rabbit, rat, chicken, and codfish. In the pig and cow, traces of the A-glycolyl derivative were also found. The novel trisialoganglioside, Giia (3), has been isolated and characterized and is responsible for 0.6% of the total ganglioside fraction of human brain. ... 

Maccioni, A. H. R., Gimenez, M. S., Caputto, B. L., and Caputto, R., 1974, Labeling of the gangliosidic fraction from brains of chickens exposed to different levels of stimulation after injection of 6- H glucosamine. Brain Res. 73 503. 

Fractions of 6 ml volume were collected and 50 yl aliquots used to identify the gangliosides by TLC. Four gangliosides have been purified from fraction I of chicken skeletal muscle directly from the column. 

Comparison of glycosphingolipids from human and chicken skeletal muscle. The elution of gangliosides from DEAE-Sephadex A 50 column with these 0.01, 0.02 and 0.2 M sodium acetate concentrations separated the gangliosides into mono-, di- and poly-sialo- fractions. The gangliosides of human muscle are shown in Fig. 1A. The monosialogangliosides GM3, GM2 and GM1 were eluted with 0.01 M sodium acetate in methanol (lane 2), GD3 and GDla with the 0.02 M solvent (lane 4) and others with 0.2 M acetate... 

Rats and chickens maintained in the dark for several days were injected with b- H-glucosamine and divided in 2 groups one remained in the dark while the other was exposed to 1000 lux. Rats were exposed to illumination for 1 h chickens for 2 h. Then animals were killed and the nervous tissue fractionated according to Eichberg et al. (1964). Gangliosides and sialic acid determinations as described previously. (Maccioni et al., 1971 Maccioni A. H. R. et al., 1974). p was calculated by Student s test for correlated data. Values for animals in the dark were taken as 100%. 

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