Cervical tissue

The uptake of TRA into cervical tissue was determined by measuring tissue radioactivity following insertion of the collagen sponge cervical cap containing tritium-labeled TRA. The TRA concentrations peaked at 4 hr and then diminished rapidly by 24 hr. Since measurements of blood samples revealed that no systemic absorption had occurred, high local concentrations over an extended period of time may be possible without systemic side effects. 

GAMBOA-PINTO A J, ROCK C L, FERRUZZI M G, SCHOWINSKY A B and SCHWARTZ S J (1998) Cervical tissue and plasma concentrations of alpha-carotene and beta-carotene in women are correlated. /iVMtr. 128(11) 1933-6. 

FTIR point-to-point mapping of cervical tissue... 

Figure 10.1 Mean extracted spectra from a cluster analysis performed on cervical tissue. The spectra were extracted from the three outermost clusters surrounding a gland that was devoid of tissue. The clusters represent, approximate distances of (A) 5 [xm, (B) lOjxmand (C)20 xm penetration into the surrounding stroma. Spectra (A) and (B) show varying degrees of die dispersion artifact while spectrum (C) is devoid of the artifact.

Figure 10.3 (a)H E stained section of cervical tissue together with a series of UHCA maps using identical multivariate and preprocessing parameters except for the number of smoothing points, (b) 5 points, (c) 7 points, (d) 9 points, (e) 11 points, (f) 13 points, (g) 15 points and (h) 17 points. 

Having shown that IR imaging and UHCA analysis is capable of extracting mean spectra for the major cell types in cervical tissue samples, there are a number of remaining goals for the cervical cancer work. First, we wish to develop a methodology to determine the optimum number of clusters in any analysis that... 

Chiriboga, L., Xie, P., Yee, H., Zarou, D., Zakim, D. and Diem, M. (1998) Infrared spectroscopy of human cells and tissues. IV. Detection of dysplastic and neoplastic changes in human cervical tissue via infrared microscopy. Cell Mol. Biol. 44, 219-29. 

TAC of homogenized cervical tissue from women with neoplasia was significantly lower than that from controls, whereas results for treated patients were intermediate between those from diseased and normal samples. Such differences were not noted for blood serum of controls and patients with cervical neoplasia (C34). 

Lin X, Paskaleva EE, Chang W et al (2011) Inhibition of HIV-1 infection in ex vivo cervical tissue model of human vagina by palmitic acid implications for a microbicide development. PLoS One 6 e24803... 

Woolfson, A. D., McCafferty, D. F., McCarron, P. A., and Price, FI. (1995), A bioadhesive patch cervical drug delivery system for the administration of 5-fluorouracil to cervical tissue, J. Controlled Release, 35,49-58. 

Often, low levels of carotenoids in biological samples provide significant challenges in quantification by HPLC-PDA alone. Electrochemical detection (ECD) has been successful in quantifying low concentrations of carotenoids (MacCrehan and Schonberger, 1987 Finckh et ah, 1995 Yamashita and Yamamoto, 1997). More information about ECD can be found in Chapter 2. ECD has also been successful in quantifying carotenoid isomers in foods, plasma, prostate tissue, cervical tissue, and buccal mucosal cells (Ferruzzi et ah, 1998,2001 Allen et ah, 2003 Unlu et ah, 2007). Electrochemical array detection for all-irans - 3-carotene has been reported to be 10 fmol on column, which is approximately 100-1000 times more sensitive than UVA is detectors (Ferruzzi et ah, 1998). 

Lycopene has been reported to increase the survival rate of mice exposed to x-ray radiation (Forssberg et al., 1959). Ribaya-Mercado et al. (1995) reported on the protective effects of lycopene toward oxidative stress-mediated damage of the human skin upon irradiation with UV light. Peng et al. (1998) examined the levels of different carotenoids, including lycopene, and vitamins A and E in plasma and cervical tissues obtained from 87 women subjects (27 cancerous, 33 precancerous and 27 noncancerous). Women with cancer had lower plasma levels of lycopene, other carotenoids, vitamin A and E compared to pre- and noncancerous women. The... 

Palozza, P. 1998. Prooxidant actions of carotenoids in biologic systems. Nutr. Rev. 56(9) 257-265. Parker, R.S. 1996. Absorption, metabolism and transport of carotenoids. FASEB J. 10 542-551. Peng, Y.M., Peng, Y.S., Childers, J.M., Hatch, K.D., Roe, D.J., Lin, Y. and Lin, P. 1998. Concentrations of carotenoids, tocopherols and retinol in paired plasma and cervical tissue of patients with cervical cancer, precancer and noncancerous diseases. Cancer Epidemiol. Biomark. Prev. 7 347-350. 

Friedrich, M., C. Villena-Heinsen, R. Axt-Fliedner, R. Meyberg, W. Tilgen, W. Schmidt et al. (2002). Analysis of 25-hydroxyvitamin Dj-la-hydroxylase in cervical tissue. Anticancer Res. 22, 183-186. 

Figure 6.5a shows the pressure dependence of the CH2 bending mode frequency of the methylene chain of lipids for both normal and malignant cervical tissue. Pressure increases this frequency because it... 

Figure 6.5a The pressure dependence of the CHj bending mode Lode frequency of the methylene chain of lipids for normal (o) and. Jand malignant ( ) cervical tissues...

Modulates cervical tissue proliferation human papilloma... 

P Greenhead, P Hayes, PS Watts, KG Laing, GE Griffin, RJ Shattock. Parameters of human immunodeficiency virus infection of human cervical tissue and inhibition by vaginal virucides. J Virol 74 5577-5586, 2000. 

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