FTIR focal plane array imaging of cervical tissue

3 FTIR focal plane array imaging of cervical tissue 

Only lysine residues on the exterior of the protein molecule are thought to react and these usually account for 40-60% of the total lysyl residues.29 Formalin does not precipitate proteins and only slightly precipitates other components of the cell it is also a good fixative for complex lipids but has no effect on neutral fats and neither preserves nor destroys adipose tissue. Formalin also traps carbohydrates because it preserves proteins so that they hold glycogen, which is otherwise readily leached from the cell. Another advantage of the compound is that it does not produce large bands that obscure the major bands associated with the macromolecules in tissue samples. Furthermore formalin is inexpensive and available in most laboratories. 

4 Stage 4 - dewaxing. The final process after the tissue sections have been deposited onto the FTIR substrate is the removal of paraffin from tissue that would 

4 Multivariate image reconstruction of FTIR array data 

The data processing can be divided into three phases. Phase 1 is the removal of poor quality spectra with an automated routine. Phase 2 is the data preprocessing of the spectra, which passed the quality test. This usually entails some type of baseline correction and normalization process. Phase 3 is multivariate image reconstruction where the spectra are classified and reproduced as color points 

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