Cervical dislocation

From largely circular colonic smooth muscle of male guinea-pigs (500—700 g killed by cervical dislocation and bleeding) single smooth muscle cells were enzymatically dissociated as previously described (McCarron Muir 1999). 

Fish. Solid biota samples, mainly fish, should be quickly killed by liquid N2 [28] or cervical dislocation [32] and kept at low temperatures (— 20°C). Some authors preferred desiccation of the sample at high temperature (70°C) [33,34] or lyophylisation [28]. The extraction and isolation steps would be combined when using lyophylisation and homogenisation, followed by a Soxhlet extraction, usually with MeOH, and a subsequent solid-phase extraction (SPE) clean-up, prior to the quantification. 

On day 8, the mice were killed by cervical dislocation and their spleens were quickly removed. CD8+ and NK1.1.+T cell populations were analyzed by flow cytometry. 

Kill immune animals by cervical dislocation or C02 inhalation, test bleed, and open abdominal cavity. Remove spleens or mesenteric nodes by blunt dissection. 

Prior to carrying out a fusion, give the mouse with the highest antibody titer a booster dose of 0.05 mL of antigen without adjuvant intraperitoneally. Three days later, kill the mouse by cervical dislocation and remove spleen aseptically. 

Kill the mouse 3 d later by cervical dislocation and remove its spleen aseptically for cell harvesting. 

In order to reduce the risk of changes in temperature and pH, oocyte collection should take no more than 5 min from euthanasia to oocyte collection (i.e., practice). Typically cervical dislocation is used to prevent possible exposure to agents that may affect oocyte or embryo quality and to reduce the time from euthanasia to oocyte collection. 

Euthanize mice at selected time intervals following infection by cervical dislocation. Remove the lungs aseptically and place them in a 10 ml homogenization tube containing 4.5 ml of sterile saline. 

Kill the mouse (6-week-old mice are best) by cervical dislocation and pin to a board. 

Haga et al. (1994) studied gastric emptying in mice. Male mice, weighing 18-22 g, had free access to food and water before the experiment. The test compounds were administered orally in 10 ml/kg 0.5 % methyl-cellulose solution. The mice were deprived of food and water and sacrificed 4 hours later by cervical dislocation. The stomachs were removed and opened. The contents of the stomach were mixed with 10% trichloroacetic acid, and centrifuged at 3000 rpm for 30 min. The weight of the sediment was taken as the food remaining in the stomach. 

Anaesthetized mice (female, SKH-1, from Simonsen, injected intraperitoneally with sodium pentobarbital, 50 mg/kg body weight) were irradiated on one flank with light from the solar simulator. Mice were killed by cervical dislocation immediately after irradiation, and irradiated skin as well as control, non-irradiated skin from the contralateral flank were removed. Adhering fat and subcutis were gently scraped free and the samples were frozen in liquid nitrogen. 

Euthanasia methodology affects the ease by which metastases can be detected and quantified. Three methods have been used by my laboratory—carbon dioxide asphyxiation, cervical dislocation and overdose using anesthetics. Alt methods for euthanizing an-imeds must be approved beforehand by the Institutional Animal Care and Use Committees (lACUC). Guidelines for euthanasia are evolving hence, regular consultation with veterinarians concerning procedures is advised. 

Mice are killed 6 h later by cervical dislocation, and excised tumors are cryo-fixed as described earlier. 

Mice are killed 72 h later by cervical dislocation, and excised tumors are fixed in a 4% buffered paraformaldehyde overnight at 4°C, blotted dry of excess paraformaldehyde and kept in 20% sucrose in PBS overnight at 4°C. 

At the end of the treatment period, sacrifice the rats by cervical dislocation and dissect liver, spleen, lungs, and kidneys. 

Rat brain cortex membrane was isolated, and the membrane homogenate was used as a preparation containing somatostatin receptors. Four rats were sacrificed by cervical dislocation. The cerebral cortex tissue was washed three times with buffer (HEPES 20niM, pH7.3, containing lOmM MgCl2) and homogenized in ice-cold buffer, using a cortex to buffer ratio of 1 10, at... 

Euthanize the tumor bearing SCID mouse by cervical dislocation. 

On day 61 after surgeiy, the rats were sacrificed by anaesthetizing them with carbon dioxide (C02) gas, followed by cervical dislocation. Immediately after the animal was dead, an incision of about 1 cm at the sample location was made using a scalpel blade and the hydrogel implants were removed with forceps. The... 

For our experimental approach, both ears of the mice were treated with acetone or the test compound in acetone 20 minutes prior to the application of TPA in acetone. At the end of the treatment period, the mice were sacrificed by cervical dislocation and the ears punched. The ear punches were then weighed, homogenized with phosphate-buffered saline, and tested for the levels of various inflammatory biomarkers. The results of these experiments are shown in tables 10.1 to 10.3. 

Male Spargue-Dawley rats (200-300 g) were killed by cervical dislocation following ether anesthesia. The abdomen was opened by midline inci-... 

After the exposure period at the specific metal concentrations, the fish from the experimental and control groups were sacrificed by cervical dislocation, weighed. 

A suspension of 2.5mg of BCG (viable bacilli) in 0.2 mL of saline is injected via the tail vein into each mouse, and 10 days later a solution of 7.5 pg of LPS in 0.2 mL of saline is injected. The mice are anesthetized with ether, sacrificed by cervical dislocation 16h after LPS injection, and trunk blood is collected into heparinized tubes (50U/mL) and centrifuged at 4°C and 1500rpm for lOmin. Serum is aspirated and stored at -70°C until assayed as described below. The liver is also removed and stored at -70°C until required. 

Allow endogenous cells to migrate for 4 h and kill mice by cervical dislocation or another lACUC-approved procedure. 

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