Cell transplantation tools

In conclusion, clonality analysis is fundamental criteria for the diagnosis of many types of lymphoma and an essential tool for the differentiation between lymphoma and other benign or unclassified hyperplasic lymphoid lesions or pseudolymphoma. Additionally clonality analysis is also useful for the screening of bone marrow before autologous bone marrow or stem cell transplantation or for the detection of minimal residual disease in bone marrow and peripheral blood. 

Fan X, Tyerman K, Ang A, et al. A novel tool for B-cell tolerance research characterization of mouse alloantibody development using a simple and reliable cellular ELISA technique. Transplant. Proc. 2005 37 29-31. 

The potential use of gene therapy has since expanded as conditions such as cancer, atherosclerosis, transplant operations, and infectious disease are now viewed as suitable targets for intervention. For example, HTV and parasitic infection (2-5). Furthermore, the ability to transfer genes into cell in vitro is also an important tool in the research of gene expression. 

Thus, cell surface markers remain a useful tool and have widespread use, as evidenced by the stem cell literature. Limitations, explained in the bone marrow transplantation literature, highlight the need for the development of new methods for identifying stem cells in the emerging field of cell therapy for cardiac diseases. Indeed, functional assays will likely play an important role in stem cell selection and classification in the future. 

Cell types of various sources and merits, such as cells derived from embryos - embryonic stem cells - from fetuses, or from tissues and organs, have been considered for cellular therapy in the CNS. Because they do not carry ethical and political concerns, and allow rewiring of the CNS, adult NSCs offer of model of choice for cellular therapy. Aside from the ethical and political concerns, NSCs offer a powerful tool for cellular therapy, particularly for cellular transplantation. 

Human cells, tissues, or organs implanted or transplanted in animals present a relatively strong and valuable tool, provided that immune rejection of the human material can be avoided. Immune-deficient animals like mice may well be used to develop organ-like structures (teratomas) from transplanted stem cells [13] or with combinations of human cells or human tissue (e.g., synoviocytes and cartilage [14], and activated mononuclear cells and transplanted skin [15]). 

Rosinski, S., Lewinska, D., Migaj, M., Wozniewicz, B., and Werynski, A., Electrostatic microencap-sulation of parathyroid cells as a tool for the investigation of cell activity after transplantation, Landbauforschung Volkenrode, SH 241, 47-50, 2002. 

The ability to transplant cells and tissue allows one to assess the interactions and behaviors of cells and tissues when placed in ectopic locations or different genetic backgrounds. The combination of microinjection with transplantation complements the genetic tools offaed by zebrafish, facilitates studies of inductive interactions and cell behaviors, and raiables the generation of gmn-line chimeras. 

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