Cell growth - chitosan film

Hamilton V et al (2007) Bone cell attachment and growth on well-characterized chitosan films. Polym Int 56(5) 241-247... 

Neat polymers have been used extensively for drug delivery applications and as biomaterials that promote cell adhesion. In fact, some of the earliest polymer scaffolds were simply films that promoted cell adhesion and growth (Ito et al., 1991). Aljawish et al. synthesized surface-modified chitosan films and studied the degree of protein adsorption and cell adhesion onto the substtate. Heterogeneous surface morphology of the films improved protein adsorption and subsequently resulted in favorable cell attachment and spreading (Aljawish et al., 2014). Film thickness also played a role in increasing cell viability. It was discovered that thicker films resulted in better cell viabihty (Aljawish et al., 2014). 

The ability of the films to support cell adhesion was evaluated by using the film as a substrate for cell growth. Films were placed into wells and coated with a cell rich solution. After seven days, all films were microscopically and histopathologically examined. Some of the results obtained are shown in Figures 4 a-d. Both the PLA and PCL films had complete monolayers on their surfaces comparable to the control. The CH film had either sparse, rounded cells or no cells present. The work by Albanese had determined that cells could not attach themselves to substrates such as EO-sterilized CH. This substrate changed the shape of the cells from the usual elongated cylindrical fibroblast form, to a rounded form and the cells then remained in suspension (34). The y-irradiated chitosan film also showed the same behavior. 

Chitosan-collagen composite Isolated aortic valve endothelial cell (VEC) cultures and preferential adhesion to fibronectin, collagen types IV and I over laminin and osteo-pontin. Chitosan-collagen type IV films act as protein precoatings The composite showed improved VEC growth and morphology in comparison to chitosan alone Certain alteration in the properties of chitosan can improve amenability to valve tissue engineering applications Cuy et al. [126]... 

Sun J, Dai ZW, Zhao Y, Chen GQ (2007) In vitro effect of oUgo hydroxyalkanoates on the growth of murine fibroblast cell line L929. Biomaterials 28 3896-3903 Suyatma NE, Copinet A, Ughzert L, Coma V (2(X)4) Mechanical and barrier properties of biodegradable films made from chitosan and poly(lactic add) blends. J Polym Environ 12 1-6... 

Aljawish, A., Munigfia, L., Jasniewski, J., Klouj, A., Scher, J., Chevalot, I., 2014. Adhesion and growth of HUVEC endothelial cells on films of enzymatically functionalized chitosan with phenolic componnds. Process Biochemistry 49, 863-871. 

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