Plasmids catabolic

Keshavarz T, MD Lilly, PC Clarke (1985) Stability of a catabolic plasmid in continuous culture. J Gen Microbiol 131 1193-1203. 

McClure NC, WA Venables (1987) pTDNl, a catabolic plasmid involved in aromatic amine catabolism in Pseudomonas putida mt-2. J Gen Microbiol 133 2012-2011. 

Lack of repeatability of the results of metabolic studies using laboratory strains that have been maintained by repeated transfer for long periods under nonselective conditions may be encountered. These strains may no longer retain their original metabolic capabilities, and this may be particularly prevalent when the strains carry catabolic plasmids that may have been lost under nonselective conditions. For these reasons, strains should be maintained in the presence of a cryoprotectant such as glycerol at low temperatures (-70°C or in liquid Nj) as soon as possible after isolation. Freeze-drying is also widely adopted, and is recommended. 

Dunn NW, HM Dunn, RA Austen (1980) Evidence for the existence of two catabolic plasmids coding for the degradation of naphthalene. J Gen Microbiol 117 529-533. 

Romine ME et al. (1999) Complete sequence of a 184-kilobase catabolic plasmid from Sphingomonas aromaticivorans. J Bacteriol 181 1585-1602. 

Park H-S, H-S Kim (2000) Identification and characterization of the nitrobenzene catabolic plasmids pNBl and pNB2 in Pseudomonas putida. J Bacterial 182 573-580. 

Sarand I, S Timonen, E-L Nurmiaho-Lassila, T Koivula, K Haatela, M Romantschuk, R Sen (1998) Microbial biofilms and catabolic plasmid harbouring degradative fluorescent pseudomonads in Scots pine mycor-rhizospheres developed on petroleum contaminated soil. FEMS Microbiol Ecol 27 115-126. 

Figure 1. Restriction fragment finger print for the 4-chlorobiphenyl catabolic plasmid pSS50 determined by agarose gel electrophoresis. (Lanes A, kHind III standard B, undigested C-H enzymatic digests of pSS50, C, Eco RI D, Bam HI E, Hind HI F, Eco M and Bam HI G, Esq RI and Hind IH H, Hind m and am HI )...

The study of catabolic plasmids involves analysis of both structural and regulatory genes and the enzymatic activities of the gene products. Catabolic plasmids that specify similar enzymatic activities often have similar restriction endonuclease fragmentation patterns, and regions of homologous DNA. 

Harayama, S., Leppik, R. A., Rekik, M., Mermod, N., Lehrbach, P.R., Reineke, W. Timmis, K. N. (1986). Gene order of the TOL catabolic plasmid upper pathway operon. Journal of Bacteriology, 167, 455-61. 

Hooper, S. W., Dockendorff, T. C. Sayler, G.S. (1989). Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50. Applied and Environmental Microbiology, 55, 1286-8. 

In a case analogous to the combination of the two plasmid encoded ortho- and mta-fission pathways in B13 for specific halo-aromatic utilization, another bacterium was modified with a hybrid pathway for this purpose. B. cepacia G4 degrades toluene, phenol, benzene, and ortho-cresol via a unique toluene ottfw-monooxygenase (Tom) pathway (Shields et al., 1989) encoded by the toluene catabolic plasmid TOM (Shields et al., 1995). 

A similar addition of a catabolic plasmid was used to construct a Pseudomonas strain capable of utilizing 2,4,6-trinitrotoluene (TNT) as a carbon and energy source (Duque et al., 1993). Pseudomonas sp. clone A utilizes the nitro-groups of TNT as the sole source of nitrogen while growing with fructose as the carbon source. Toluene is produced as a consequence of this activity, which cannot be metabolized by clone A. Introduction ofTOLallowed complete utilization of TNT without the need for an ancillary source of carbon and energy. 

Studies designed to test the mobilization and persistence of recombinant catabolic plasmid pDlO. 

Flarayama, S. Don, R. H. (1985). Catabolic plasmids their analysis and utilization in the manipulation of bacterial metabolic activities. In Genetic Engineering Principles and Methods, Vol. 7, ed. J. K. Setlow and A. Hollaender, pp. 283-307. New York Plenum Publishing Co. 

Martinez, B., J. Tomkins, L.P. Wackett, R. Wing, and MJ. Sadowsky (2001). Complete nucleotide sequence and organization of the atrazine catabolic plasmid pADP-1 from Pseudomonas spp. strain ADP. J. Bacteriol., 183(19) 5684—5697. 

Gunsalus laboratory, the P450(, system allowed biochemical and biophysical investigation of the CYP catalytic cycle as well as of the genetics of a bacterial catabolic plasmid. This typical CYP system was found to require a ferredoxin and ferredoxin reductase for catalytic activity, unlike the model for eukaryote CYPs, CYP102A1 or P450g 3, which was discovered in the Fulco laboratory and consisted of a fusion polypeptide containing CYP and reductase domains. ... 

Fernandez, M., Niqui-Arroyo, J.L., Conde, S., Ramos, J.L., and Duque, E. (2012) Enhanced tolerance to naphthalene and enhanced rhizoremediation performance for Pseudomonas putida KT2440 via the NAH7 catabolic plasmid. Appl. Environ. Microbiol., 78 (15), 5104-5110. 

Figure 2. A) Genetic organization of the pKAl catabolic plasmid. Genes of the upper naphthalene regulatory system encode for proteins that mediate the conversion of naphthalene to salicylate. Salicylate is then further degraded to TCA cycle intermediates. B) In Pseudomonas fluorescens HK44, genes within the lower pathway were replaced with genes of the lux cassette to produce a bioluminescent bioreporter sensitive to naphthalene and salicylate.

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