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Carbodiimides carboxylates

The available free carboxyl groups of the DAS—HMS can be linked via a peptide bond to available primary amine groups onto highly antigenic carriers using a carbodiimide (19). The carriers used in this case were bovine semm albumin (BSA) and poly-L-lysine (molecular weight 150,000 to 300,000). The... [Pg.24]

Anhydride Formation. The carboxyl group ia A/-protected amino acids is converted iato the symmetrical anhydride on treatment with the carbodiimide (84). [Pg.281]

An intramolecular Diels-Alder cyclization produces excellent yields of 2-aminoquinoline-3-carboxylate esters (57). Equally fine yields of the requited carbodiimides have been reported, making this an attractive route to an unusual substitution type. [Pg.392]

Molecules of interest that contain free amino groups can be coupled in aqueous solution to /S-poIy(L-malate) as amides using carbodiimides such as the water-soluble l-ethyl-3(3-dimethyIaminopropyl)carbodiimide hydrochloride (EDC) [2,12,20,21]. By this method, the molecules are attached randomly. A selective amide bond formation at the carboxylate terminus can be achieved... [Pg.99]

Mechanism of amide formation by reaction of a carboxylic acid and an amine with dicyclohexyl-carbodiimide (DCC). [Pg.798]

Activating agents, such as trifluoroacetic anhydride 1,1 -carbonyldiimidazolc carbodiimides sulfonyl, tosyl, and picryl chlorides and a range of phosphorus derivatives can promote direct solution reactions between dicarboxylic acids and diols or diphenols in mild conditions. The activating agents are consumed during the reaction and, therefore, do not act as catalysts. These so-called direct polycondensation or activation polycondensation reactions proceed via the in situ transformation of one of the reactants, generally the carboxylic acid, into a more... [Pg.77]

The reaction between acyl halides and diazomethane is of wide scope and is the best way to prepare diazo ketones. Diazomethane must be present in excess or the HX produced will react with the diazo ketone (10-74). This reaction is the first step of the Amdt-Eistert synthesis (18-8). Diazo ketones can also be prepared directly from a carboxylic acid and diazomethane or diazoethane in the presence of dicyclohexyl-carbodiimide. ... [Pg.573]

Reduction of uronic acids Uronic acids (UA) were converted to the corresponding neutral sugars (NS) by carbodiimide activation of the carboxyl groups followed by a reduction with NaBD4 according to the method of Kim and Carpita [5]. In order to achieve a complete reduction of the uronic acids the procedure was repeated once. [Pg.652]

PGIP, purified fi om P.vulgaris hypocotyls [11], was immobilized to the sensor ch via amine coupling. A continuous flow of HBS buffer (5 pl/min) was mantained over the sensor surface. The carboxylated dextran matrix of the sensor surface was first activated by a 6-min injection of a mixture of N-hydroxy-succinimide and N-ethyl-N - (3-diethylaminopropyl) carbodiimide, followed by a 7-min injection of PGIP (lOng/pl in 10 mM acetate, pH 5.0). Hie immobilization procedure was con leted by a 7-min injection of 1 M ethanolamine hydrochloride to block the remaining ester groups. [Pg.776]

The reaction of Ccf -ATPase with dicyclohexylcarbodiimide Carbodiimides readily react in aqueous solutions with protein amino, carboxyl and sulfhydryl groups slower reactions with tyrosine and serine have also been reported [369,370]. The primary reaction product of carboxyl groups with dicyclohexylcarbodiimide is dicyclohexyl-O-acyl isourea [370]. Dicyclohexyl-O-acyl isourea is susceptible to nucleophilic attack either by water or by endogenous or exogenous nucleophiles, yielding a complex series of reaction products [369-371]. [Pg.96]

Figure 15 Conjugation of a carboxylic add and an amine using the carbodiimide method. The carbodiimide activates the carboxyhc acid to speed up the reaction to the amine. Carbodiimides can be used with nonpolar or polar solvents, including water. Undesirable urea complexes may form as by-products. Details of the reaction are given in Table 3... Figure 15 Conjugation of a carboxylic add and an amine using the carbodiimide method. The carbodiimide activates the carboxyhc acid to speed up the reaction to the amine. Carbodiimides can be used with nonpolar or polar solvents, including water. Undesirable urea complexes may form as by-products. Details of the reaction are given in Table 3...
Figure 9 A synthetic mixture of water-soluble carboxylic acids separated by anion-exchange chromatography. Column 0.3 cm x 300 cm Diaoion CA 08, 16-20 p (Mitsubishi Kasei Kogyo). Eluant 200 mM HC1. Detection reaction with Fe3-benzohy-droxamic acid-dicyclohexy carbodiimide-hydroxylamine perchlorate-triethyl amine with absorbance at 536 nm. Analytes (1) aspartate, (2) gluconate, (3) glucuronate, (4) pyroglutamate, (5) lactate, (6) acetate, (7) tartrate, (8) malate, (9) citrate, (10) succinate, (11) isocitrate, (12) w-butyrate, (13) a-ketoglutarate. (Reprinted with permission from Kasai, Y., Tanimura, T., and Tamura, Z., Anal. Chem., 49, 655, 1977. 1977 Analytical Chemistry). Figure 9 A synthetic mixture of water-soluble carboxylic acids separated by anion-exchange chromatography. Column 0.3 cm x 300 cm Diaoion CA 08, 16-20 p (Mitsubishi Kasei Kogyo). Eluant 200 mM HC1. Detection reaction with Fe3-benzohy-droxamic acid-dicyclohexy carbodiimide-hydroxylamine perchlorate-triethyl amine with absorbance at 536 nm. Analytes (1) aspartate, (2) gluconate, (3) glucuronate, (4) pyroglutamate, (5) lactate, (6) acetate, (7) tartrate, (8) malate, (9) citrate, (10) succinate, (11) isocitrate, (12) w-butyrate, (13) a-ketoglutarate. (Reprinted with permission from Kasai, Y., Tanimura, T., and Tamura, Z., Anal. Chem., 49, 655, 1977. 1977 Analytical Chemistry).
Reactions of 2,3-dioxo-l,2,3,5,6,7-hexahydropyrido[l,2,3-carboxylic acids and the homologous acetic and propionic acids, prepared by basic hydrolysis of the corresponding ester, with amines, 28% NH4OH, and hydroxylamine derivatives in the presence of l-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and hydroxybenztria-zole <1995BML1527>, 1995BML1533>, and in the presence of NEt3 and A, A -bis(2-oxo-3-oxazolidinyl)phosphinic... [Pg.134]

The carbodiimide of choice used to couple cystamine to carboxylate- or phosphate-containing molecules is most often the water-soluble carbodiimide, EDC hydrochloride Chapter 3, Section 1.1). This reagent rapidly reacts with carboxylates or phosphates to form an active ester intermediate, which is highly reactive toward primary amines. The reaction is efficient from pH 4.7 to 7.5, and a variety of buffers may be used, providing they don t contain competing groups. [Pg.84]

Figure 1.71 Cystamine may be used to label protein carboxylate groups using the water-soluble carbodiimide EDC. Figure 1.71 Cystamine may be used to label protein carboxylate groups using the water-soluble carbodiimide EDC.
Molecules modified with succinic anhydride to create terminal carboxylate functionalities may be further conjugated to amine-containing molecules by use of amide bond forming reagents such as carbodiimides (Chapter 3, Section 1). [Pg.105]

Figure 1.88 The maleimide group of BMPA reacts with a thiol-containing molecule to result in a modification having a terminal carboxylate group. Amine-containing molecules then can be conjugated to the carboxylate using a carbodiimide reaction with EDC. Figure 1.88 The maleimide group of BMPA reacts with a thiol-containing molecule to result in a modification having a terminal carboxylate group. Amine-containing molecules then can be conjugated to the carboxylate using a carbodiimide reaction with EDC.

See other pages where Carbodiimides carboxylates is mentioned: [Pg.487]    [Pg.798]    [Pg.53]    [Pg.218]    [Pg.128]    [Pg.49]    [Pg.506]    [Pg.353]    [Pg.285]    [Pg.252]    [Pg.435]    [Pg.639]    [Pg.640]    [Pg.153]    [Pg.136]    [Pg.63]    [Pg.566]    [Pg.57]    [Pg.125]    [Pg.466]    [Pg.32]    [Pg.453]    [Pg.343]    [Pg.364]    [Pg.260]    [Pg.262]    [Pg.330]    [Pg.38]    [Pg.39]    [Pg.255]    [Pg.43]    [Pg.84]    [Pg.87]   
See also in sourсe #XX -- [ Pg.176 ]




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Carbodiimid

Carbodiimide

Carbodiimide coupling carboxylate-modified

Carbodiimides carboxylic acid amides

Carbodiimides reacting with carboxylic acids

Carbodiimids

Carboxylic acids carbodiimides

Carboxylic reaction with carbodiimides

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