Uronic acids liquid chromatography

In the carbohydrate series, the acids encountered are aldonic, aldaric, uronic, or saccharinic acids. Often, these acids are readily transformed into their lactones or methyl esters, and it is as derivatives of these that they are commonly studied. Table VIII (see p. 136) records examples in which derivatives of such acids and lactones have been subjected to gas-liquid chromatography. 

Uronic acids have been studied similarly in connection with pectins384,523 and oligogalacturonic acids,170 fruits,319 and plants.66"524 Neukom and coworkers have studied the behavior of uronic acid and also aldaric acid derivatives.525 Ascorbic acid and its analogs526 have been separated by gas-liquid chromatography and determined291 in plant extracts. 

Chromatographic methods have also been developed for quantitative determination of the AUC of pectin. Uronic acids have been analyzed using gas-liquid chromatography (GLC) after conversion to L-galactono-1,4-lactone via potassium borohydride reduction and lactonization using methanolic hydrogen chloride. The derived lactone was then analyzed by GLC as the trimethylsilyl derivative (Perry... 

Monosaccharides, oligosaccharides, uronic acid, especially o-glucose, D-fructose, sucrose Indirect UV detection D A = 256nm C Fused silica (90 cm x 50 pm i.d.) E 6 mmol r potassium sorbate, pH 12.2 V 20 kV Determination of sugars in fruit juices comparison of the results to those of liquid chromatography methods... 

Honda, S. Suzuki, S. Takahashi, M. Kakehi, K. Ganno, S. Automated analysis of uronic acids by high-performance liquid chromatography with photometric and fluorimetric... 

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