Capillary electrophoresis with flame photometric detection

Capillary electrophoresis with flame photometric detection (CE/FPD) (10). 

Capillary Electrophoresis with Flame Photometric Detection Chemical Weapons Convention Extracted Ion Chromatogram Electron Impact Mass Spectrometry Electrospray Ionization Flow Injection Analysis Flame Photometric Detector Gas Chromatography/Fourier Transform Infrared Spectroscopy Gas Chromatography/Mass Spectrometry Gas chromatography International Union for Pure and... 

E.W. Hooijschuur, C.E. Kientz and U.A. Brink-man, Application of microcolumn liquid chromatography and capillary electrophoresis with flame photometric detection for the screening of degradation products of chemical warfare agents in water and soil, J. Chromatogr. A, 928, 187-199 (2001). 

C.E. Kientz, E.W.J. Hooijschuur and U.A.Th. Brinkman, Capillary electrophoresis coupled online with flame photometric detection determination of alkylphosphonic acids, J. Microcol. Sep., 9, 253-259 (1997). 

Capillary electrophoresis (CE) is a separation technique for ionic or ionizable compounds. CE is particularly attractive because the instrumentation is inexpensive and separations are quick and efficient. As with GC and LC, CE can be coupled to and flame photometric detection (FED) to detect alkylphosphonic acids [30-32]. Indirect UV absorbance detection with CE has also been used for the analysis of nerve agents and their degradation products [33]. In an attempt to meet the demands of portable and efficient field instruments, miniaturized analytical systems with CE microchips have also been made for the separation and detection of alkylphosphonic nerve agents [34]. The aforementioned CE procedures all provide rapid identification without extensive sample preparation. CE is most likely to be used as a guide in order to select the appropriate methods for further analysis by more definitive techniques such as GC-MS, as most of the products detected and analysed are degradation products [35]. A review depicting various CE separation techniques, lab-on-a-chip technology and detection limits has been compiled by Pumera and is shown in Table 3.1. 

The separation performance and sensitivity of a capillary electrophoresis system coupled to a phosphorous-specific flame photometric detector (FPD) has been reported for the detection of alkylphosphonic acids l20 221. The liquid junction used to decouple the electric field from the FPD showed a negligible influence on the performance of the system as compared with online UV detection. The use of an on-column sample stacking preconcentration technique allowed for injection of 900 nL. With the large injection, the detection limits for the alkylphosphonic acids in water were 0.1-0.5 xgmL 1. 

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