Capillary electrochromatography method development

Euerby, M.R., et al., Solvent and stationary phase selectivity in capillary electrochromatography method development Comparison of Cl8, C8, and phenyl-bonded phases for the separation of a series of substituted barbiturates, J. Microcol. Sep., 11, 305, 1999. 

Electromigration and chromatographic methods developed as separate techniques over many decades. Today both methods have converged into a single approach capillary electrochromatography. The approach is still under development, but has already demonstrated to be very promising. From the current findings and the overall CE expertise that was... 

Zheng, J., and Shamsi, S. A. (2003). Combination of chiral capillary electrochromatography with electrospray ionization mass spectrometry method development and assay of warfarin enantiomers in human plasma. Anal. Chem. 75, 6295—6305. 

The developments of the Lewis base-modified zirconia and mixed-oxide containing zirconia as stationary phases for high-performance liquid chromatography (HPLC) are reviewed. In this context, the preparation methods of porous spherical zirconia, and zirconia supports for HPLC based on modification with fluoride, phosphate, phos-phonate, carboxylic acid, phenols, and protein, as well as cyclodextrin derivative, are covered. The application of modified-zirconia in capillary electrochromatography (CEC) is also discussed. 

To control the extent of water contamination with substances of environmental concern it is necessary to develop rapid and sensitive analytical methods. Modem gas chromatography (GC) and Hquid chromatography, especially in combination with mass spectrometry (MS), capillary electrophoresis (CE), and capillary electrochromatography are the most suitable techniques for the analysis of both an individual component and a complex mixture. However, as a mle, the concentration of solutes to be analyzed in water samples is too low, in the range of nanograms to micrograms per liter, to inject a probe directly into a chromatographic analytical column and, therefore, pre-enrichment of water samples is required. 

M.C. Breadmore, A.S. Palmer, M. Curran, M. Macka, N. Avdalovic and PR. Haddad, On-column ion-exchange of inorganic anions in open tubular capillary electrochromatography with elution using transient-isotachophoresis gradients. 3. Implementation and method development. Anal. Chem., 74, 2112-2118, 2002. 

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